An analysis of the feasibility of short read sequencing
| Autor: | Nava E. Whiteford, Gerald Weber, Peter L. Roach, Jonathan W. Essex, Mark Bradley, Cameron Neylon, Niall J. Haslam, Adam Prügel-Bennett |
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| Rok vydání: | 2005 |
| Předmět: |
Genetics
Contig CHIP Genome Human Shotgun sequencing Sequence assembly Hybrid genome assembly DNA Genome Viral Genomics Sequence Analysis DNA Biology HIGH-THROUGHPUT Deep sequencing DNA sequencing ARRAYS Chromosomes Human Pair 1 Primer walking HUMAN GENOME Feasibility Studies Humans Methods Online Genome Bacterial Paired-end tag |
| Zdroj: | Nucleic Acids Research Whiteford, N, Haslam, N, Weber, G, Prugel-Bennett, A, Essex, J W, Roach, P L, Bradley, M & Neylon, C 2005, ' An analysis of the feasibility of short read sequencing ', Nucleic Acids Research, vol. 33, no. 19, E171, pp.-. https://doi.org/10.1093/nar/gni170 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1. |
| Databáze: | OpenAIRE |
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