Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing
| Autor: | Dongwei Huang, Ding Haisheng, Huiling Zhao, Xiaofei Wang, Xiaowei Zhao, Yunxia Qi |
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| Rok vydání: | 2021 |
| Předmět: |
lcsh:QH426-470
lcsh:Biotechnology Histological analysis Biology 03 medical and health sciences 0302 clinical medicine Transcription (biology) lcsh:TP248.13-248.65 Genetics medicine Animals Hedgehog Proteins Gene Skin 030304 developmental biology 0303 health sciences LncRNA expression Sequence Analysis RNA Activator (genetics) Gene Expression Profiling RNA RNA sequencing Lipid metabolism Hair follicle Long non-coding RNA Cell biology lcsh:Genetics medicine.anatomical_structure 030220 oncology & carcinogenesis Female RNA Long Noncoding Angora rabbit Rabbits Wool production Janus kinase Hair Follicle Hair Research Article Hair follicle density Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 22, Iss 1, Pp 1-10 (2021) |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-021-07398-4 |
| Popis: | Background Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan Strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive. Results In this study, hair follicle density at different body sites of Wan Strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR). Conclusions LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density. |
| Databáze: | OpenAIRE |
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