Patterns and dynamics of subventricular zone neuroblast migration in the ischemic striatum of the adult mouse
| Autor: | Longfei Jia, Benjamin Buller, Zheng G Zhang, Sara R Gregg, Yier Toh, Rui L Zhang, Siamak P Nejad Davarani, Yvonne LeTourneau, Cindi Roberts, Michael Chopp |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Genetically modified mouse
animal structures Doublecortin Protein Subventricular zone Striatum Article Brain Ischemia Cerebral Ventricles Brain ischemia Mice Neuroblast Cell Movement Neuroblast migration medicine Animals reproductive and urinary physiology Neurons Microscopy Video biology Stem Cells fungi medicine.disease Corpus Striatum Doublecortin Stroke Kinetics medicine.anatomical_structure Neurology nervous system Cerebrovascular Circulation biology.protein Neurology (clinical) Cardiology and Cardiovascular Medicine Neuroscience Ganglion mother cell |
| Popis: | The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster ( P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes. |
| Databáze: | OpenAIRE |
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