New cellular models for tracking the odontoblast phenotype
| Autor: | Juliette Bitard, Morgane Locker, Mireille Bonnefoix, V. Ronco, Anne Poliard, F. Priam, Michel Goldberg, T. Duchêne, K. Bourd, T. Wurtz, Odile Kellermann |
|---|---|
| Přispěvatelé: | Faculté de Chirurgie dentaire de Montrouge, Génétique moléculaire et intégration des fonctions cellulaires (GMIFC), Centre National de la Recherche Scientifique (CNRS), Régulations et communications cellulaires (RCC (IFR_58)), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
MESH: Odontoblasts
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Cell Culture Techniques Odontoblast differentiation Cell Separation Simian virus 40 Mice 0302 clinical medicine MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Animals 0303 health sciences Odontoblasts [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior Reverse Transcriptase Polymerase Chain Reaction DLX2 [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences MESH: Adenoviridae General Medicine MESH: Genes medicine.anatomical_structure Dentin sialoprotein MESH: Dental Pulp MESH: Mice Transgenic Recombinant Fusion Proteins Mesenchyme MESH: Tooth Germ Blotting Western Mice Transgenic Biology MESH: Cell Separation Adenoviridae Viral Proteins 03 medical and health sciences MESH: Gene Expression Profiling stomatognathic system MESH: Simian virus 40 medicine MESH: Recombinant Fusion Proteins Animals MESH: Blotting Western General Dentistry MESH: Mice Dental Pulp 030304 developmental biology MESH: Cell Culture Techniques MESH: Osteoblasts Osteoblasts Gene Expression Profiling MESH: Clone Cells Tooth Germ 030206 dentistry Cell Biology Molecular biology MESH: Viral Proteins DMP1 Clone Cells stomatognathic diseases Odontoblast Genes Otorhinolaryngology MESH: Biomarkers Pulp (tooth) Homeobox Biomarkers |
| Zdroj: | Archives of Oral Biology Archives of Oral Biology, Elsevier, 2005, 50 (2), pp.271-277. ⟨10.1016/j.archoralbio.2004.10.007⟩ |
| ISSN: | 0003-9969 |
| Popis: | International audience; Odontoblasts and osteoblasts differ functionally and histologically. Because of their close relationship, mesenchymal cells derived from teeth and bone are difficult to distinguish ex vivo. Indeed, the main non-collagenous components of the odontoblastic extracellular matrix, dentin sialoprotein (DSP) or dentin matrix protein 1 (DMP1), have also been detected in osteoblasts. The need to develop cellular models of odontoblast differentiation and to identify markers specific for the odontoblast lineage, has led us to establish clonal cell lines from tooth germs of day 18 mouse embryos transgenic for an adenovirus-SV40 recombinant plasmid. In this study, we analyzed the phenotypes of three independent clones by RT-PCR and Western blot. These clones synthesised DSP, DMP1 and other extracellular matrix proteins typical of the odontoblast and are therefore likely to be derived from the pulp. Transcripts encoding a set of homeobox proteins involved in craniofacial development, such as Pax9, Msx1, Cbfa1, Dlx2 and 5 were also expressed albeit at a different level. These features of the pulpal clones are shared by the C1 mesodermal cells that are capable of differentiating along osteogenic, chondrogenic or adipogenic lineages In contrast, transcripts for two LIM-domain homeobox family genes (Lhx6 and Lhx7) were only detected in the dental clones. Since these genes are preferentially expressed in the mesenchyme of the developing tooth, this suggests that our transgenic-derived cell lines retain intrinsic properties of odontoblastic cells. They may help to characterise genes specifying the odontoblast phenotype and the signalling pathways underlying odontoblast differentiation. |
| Databáze: | OpenAIRE |
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