Rapid fabrication of precise high-throughput filters from membrane protein nanosheets
| Autor: | Yuxuan Dai, Laxmicharan Samineni, William A. Phillip, Dibakar Bhattacharya, Woochul Song, Janna N. Sloand, Scott H. Medina, Ratul Chowdhury, Enrique D. Gomez, Manish Kumar, Robert J. Hickey, Andrey Parshin, Mariusz Grzelakowski, Yu-Ming Tu, Arwa Mukthar, Alina Thokkadam, Prasangi Rajapaksha, Yue-xiao Shen, Tyler E. Culp, Chao Lang, Miaoci Zhang, Drew Carson, Yinai Wei, Tingwei Ren |
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| Rok vydání: | 2020 |
| Předmět: |
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Molecular Time Factors Materials science Low protein 02 engineering and technology 010402 general chemistry 01 natural sciences Permeability General Materials Science Nanosheet Mechanical Engineering Membrane Proteins Membranes Artificial Biological membrane General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Nanostructures 0104 chemical sciences Membrane Chemical engineering Mechanics of Materials Permeability (electromagnetism) Solvents Protein Conformation beta-Strand Self-assembly 0210 nano-technology Selectivity Hybrid material Porosity |
| Zdroj: | Nature Materials. 19:347-354 |
| ISSN: | 1476-4660 1476-1122 |
| Popis: | Biological membranes are ideal for separations as they provide high permeability while maintaining high solute selectivity due to the presence of specialized membrane protein (MP) channels. However, successful integration of MPs into manufactured membranes has remained a significant challenge. Here, we demonstrate a two-hour organic solvent method to develop 2D crystals and nanosheets of highly packed pore-forming MPs in block copolymers (BCPs). We then integrate these hybrid materials into scalable MP-BCP biomimetic membranes. These MP-BCP nanosheet membranes maintain the molecular selectivity of the three types of β-barrel MP channels used, with pore sizes of 0.8 nm, 1.3 nm, and 1.5 nm. These biomimetic membranes demonstrate water permeability that is 20–1,000 times greater than that of commercial membranes and 1.5–45 times greater than that of the latest research membranes with comparable molecular exclusion ratings. This approach could provide high performance alternatives in the challenging sub-nanometre to few-nanometre size range. Protein channels are highly selective, but application in membranes is limited due to low protein content. Here, protein channels are embedded into block copolymers to form nanosheets using rapid solvent casting, with better water permeability and similar molecular exclusions relative to other membrane systems. |
| Databáze: | OpenAIRE |
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