In Endothelial Cells, the Activation or Stimulation of Soluble Guanylyl Cyclase Induces the Nitric Oxide Production by a Mechanism Dependent of Nitric Oxide Synthase Activation
| Autor: | Thiago Francisco De Moraes, Gerson Jhonatan Rodrigues, Carla Nascimento dos Santos Rodrigues, Ariane Migliato Martinelli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male NOS inhibitor Endothelium Pharmaceutical Science lcsh:RS1-441 Stimulation Pharmacology Nitric Oxide Nitric oxide lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship 0302 clinical medicine Soluble Guanylyl Cyclase medicine Human Umbilical Vein Endothelial Cells Animals Humans ortho-Aminobenzoates No production Rats Wistar Cyclic GMP Calcimycin Sulfonamides biology Dose-Response Relationship Drug Chemistry lcsh:RM1-950 Rats Nitric oxide synthase 030104 developmental biology medicine.anatomical_structure lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis biology.protein cardiovascular system Nitric Oxide Synthase Soluble guanylyl cyclase Myograph |
| Zdroj: | Journal of Pharmacy & Pharmaceutical Sciences, Vol 21 (2018) |
| ISSN: | 1482-1826 |
| Popis: | Purpose. In endothelial cells, investigate if the soluble guanylate cyclase (sGC) activation or stimulation is able to potentiate the relaxation in vessels. Methods. Aortic and coronary rings with and without endothelium were placed in a myograph and cumulative concentration-effect curves for DETA-NO or ataciguat were performed. Nitric oxide (NO) were measured by fluorescence or by selective electrode in human umbilical endothelial cells ( HUVECs) in response to some treatments, including ataciguat, 8-Br-cGMP and A23187. Results. The presence of the endothelium potentiated the relaxation induced by DETA-NO in aortic and coronary rings. In addition, in aortic rings the endothelium potentiated the relaxation induced by ataciguat. In the presence of nitric oxide synthase (NOS) inhibitor, the endothelium effect was abolished to DETA-NO or ataciguat, in both vessels. Ataciguat, 8-Br-cGMP and A23187 were able to induce NO production in HUVECs cells. In the presence of NOS inhibitor, the NO production induced by ataciguat and 8-Br-cGMP was abolished. Conclusions. Our results suggest that in aortic and coronary rings the endothelium potentiates the relaxation induced by activation or stimulation of sGC through a mechanism dependent of NOS activation. This article is open to POST-PUBLICATION REVIEW . Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|