Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line
| Autor: | María Laura Gertiser, Verónica Poggio, Claudia Hernandez, Jorge Gentile, Alicia Graciela Fuchs, Oscar Jensen, Gabriela A. Canziani, Andrea Maglioco, Melisa S. Barbery Venturi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male 030231 tropical medicine Immunology Blotting Western Antibodies Helminth Enzyme-Linked Immunosorbent Assay Biology Serology Microbiology Cell Line 03 medical and health sciences Mice 0302 clinical medicine Blood serum Antigen Echinococcosis medicine Animals Humans Echinococcus granulosus Mice Inbred BALB C Sheep Plant Extracts Middle Aged biology.organism_classification medicine.disease Blood proteins 030104 developmental biology Blood chemistry Parasitology Antigens Helminth Case-Control Studies Female |
| Zdroj: | Parasite immunologyREFERENCES. 41(12) |
| ISSN: | 1365-3024 |
| Popis: | Cystic echinococcosis (CE) can be diagnosed by means of several serological approaches, but their results vary among laboratories due to the molecular characteristics of the reference antigens used. Thus, this study aimed to address both the relevance of an EGPE cell line previously obtained from Echinococcus granulosus protoscoleces G1 and the complexity of the immune response by using two different in vitro growth stages as separate sources of parasite antigens. The serum reactivity was investigated by western blotting (WB) in 21 CE patients from an endemic area in a matched case-control design and also in seven experimentally infected sheep and five healthy control sheep. EGPE-antigen-human serum sensitivity by WB was higher than that of hydatid fluid (HF) WB, ELISA and DD5 (P |
| Databáze: | OpenAIRE |
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