Multilocus sequence analysis reveals three distinct populations of ‘Candidatus Phytoplasma palmicola’ with a specific geographical distribution on the African continent
| Autor: | Fabian Pilet, Isaac Jesuorobo Osagie, Robert N. Quaicoe, Marcos Freire, Xavier Foissac |
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| Přispěvatelé: | Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Oil Palm Research Institute (CSIR-OPRI), Partenaires INRAE, Nigerian Institute for Oil Palm Research (NIFOR), nstituto de Investigaçao Agraria de Moçambique(IIAM), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Sequence analysis Phytopathology and phytopharmacy education Zoology CLYD 01 natural sciences Applied Microbiology and Biotechnology Agent pathogène 03 medical and health sciences mollicute phytopathogène noix de coco phytoplasme santé des plantes Candidatus Phytoplasma palmicola maladie du jaunissement mortel de la noix de coco pathologie végétale Lethal yellowing Cocos nucifera 030304 developmental biology H20 - Maladies des plantes bactérie phytopathogène 0303 health sciences Genetic diversity Ecology biology Haplotype Microbiology and Parasitology food and beverages 15. Life on land biology.organism_classification Phytopathologie et phytopharmacie Microbiologie et Parasitologie Housekeeping gene [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Phytoplasma épidémiologie Multilocus sequence typing 010606 plant biology & botany Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2019, 85 (8), pp.e02716-18. ⟨10.1128/AEM.02716-18⟩ Applied and Environmental Microbiology 8 (85), e02716-18. (2019) Applied and Environmental Microbiology, 2019, 85 (8), pp.e02716-18. ⟨10.1128/AEM.02716-18⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.02716-18⟩ |
| Popis: | To sustain epidemiological studies on coconut lethal yellowing disease (CLYD), a devastating disease in Africa caused by a phytoplasma, we developed a multilocus sequence typing (MLST) scheme for 'Candidatus Phytoplasma palmicola' based on 8 housekeeping genes. At the continental level, eight different sequence types were identified among 132 'Ca. Phytoplasma palmicola-' infected coconuts collected in Ghana, Nigeria and Mozambique where CLYD epidemics are still very active. 'Ca. Phytoplasma palmicola' appeared to be a bacterium submitted to strong bottlenecks, reducing the fixation of positively selected beneficial mutations into the bacterial population. This phenomenon, as well as a limited plant host range, could explain the observed country-specific distribution of the eight haplotypes. As an alternative means to increase fitness, bacteria can also undergo genetic exchange; however, no evidence for such recombination events were found for 'Ca. Phytoplasma palmicola'. The implications for CLYD epidemiology and prophylactic control are discussed. The usefulness of seven housekeeping genes to investigate the genetic diversity in the genus 'Candidatus Phytoplasma' is underlined. IMPORTANCE: Coconut is an important crop for both industry and small stakeholders in many intertropical countries. Phytoplasma-associated lethal yellowing-like diseases have become one of the major pests that limit coconut cultivation as they have emerged in different parts of the world. We developed a multilocus sequence typing scheme for tracking epidemics of 'Ca. Phytoplasma palmicola', which is responsible for coconut lethal yellowing disease (CLYD) on the African continent. MLST analysis applied to diseased coconut samples collected in western and eastern African countries also showed the existence of three distinct populations of 'Ca. Phytoplasma palmicola' with low intrapopulation diversity. The reasons for the observed genetic bottlenecks resulting in strong geographic patterns remain to be established but could result from the lethality of CLYD and dominance of short distance insect-mediated transmission in its spread. |
| Databáze: | OpenAIRE |
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