Upregulation of microRNA-93-5p/microRNA-4668-5p, and promoter methylation of matrix metalloproteinase-3 and interleukin-16 genes in Turkish patients with rheumatoid arthritis
| Autor: | Zülfinaz Betül Çelik, Sengul Tural, Nurten Kara, Gamze Alayli, Ahmet Kıvanç Cengiz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
medicine.medical_specialty Methylation Pathogenesis 03 medical and health sciences 0302 clinical medicine Rheumatology Downregulation and upregulation Internal medicine microRNA Medicine 030212 general & internal medicine Rheumatoid arthritis Gene 030203 arthritis & rheumatology MMP-3 business.industry MicroRNA medicine.disease IL-16 Endocrinology DNA methylation Interleukin 16 business lcsh:RC581-607 |
| Zdroj: | Egyptian Rheumatologist, Vol 43, Iss 1, Pp 35-39 (2021) |
| ISSN: | 1110-1164 |
| Popis: | Aim of the work: To investigate promoter methylation of matrix metalloproteinase-3 (MMP-3) and interleukin-16 (IL-16) genes with the expression of miRNA-93-5p and miRNA-4668-5p which target these genes in rheumatoid arthritis (RA), respectively. Patients and methods: The study included 49 RA patients and 38 healthy controls. Promoter methylation of MMP-3 and IL-16 was analyzed by methylation-specific PCR. The expression of miRNA-93-5p and miRNA-4668-5p were determined. Disease activity score (DAS28) was assessed. Results: The 49 patients (38 female, 11 male) mean age was 50.4 ± 10.5 years and disease duration of 9.1 ± 7.4 years. The mean DAS28 was 3.9 ± 1.4. The MMP-3 gene methylation frequency was significantly lower in patients (n = 37;75.5%) compared to control (n = 37;97.4%) (p = 0.004) while they were comparable for IL-16 gene (n = 46;93.9% vs n = 37;97.4%)(p = 0.45). The relative normalized expression of miRNA-93-5p and miRNA-4668-5p were significantly increased (p |
| Databáze: | OpenAIRE |
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