Cellular and molecular effects of pulsed dye laser and local narrow-band UVB therapy in psoriasis

Autor: Emőke Rácz, H. A. Martino Neumann, Jaap de Leeuw, Marius Kant, Errol P. Prens, Ewout M. Baerveldt, Leslie van der Fits
Přispěvatelé: Dermatology
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Pathology
Biopsy
Low-Level Light Therapy/methods
Biomarkers/analysis
Severity of Illness Index
Tumor Necrosis Factor-alpha/analysis
E-Selectin/analysis
Interleukin 23
Needle
Prospective Studies
Vascular Endothelial Growth Factor Receptor-1/analysis
Lasers
Dye/therapeutic use
integumentary system
medicine.diagnostic_test
biology
Biopsy
Needle
Statistics
Interleukin
Middle Aged
Immunohistochemistry
medicine.anatomical_structure
Treatment Outcome
Ultraviolet Therapy/methods
Tumor necrosis factor alpha
Ultraviolet Therapy
Female
E-Selectin
Adult
medicine.medical_specialty
T cell
Lasers
Dye
Down-Regulation
Dermatology
Vascular Endothelial Growth Factor Receptor-2/analysis
Statistics
Nonparametric
Young Adult
Psoriasis
Interleukin-23 Subunit p19/analysis
E-selectin
medicine
Humans
Nonparametric
Low-Level Light Therapy
Molecular Biology
Aged
Probability
Vascular Endothelial Growth Factor Receptor-1
business.industry
Tumor Necrosis Factor-alpha
Lasers
Dye/therapeutic use
Cell Biology
medicine.disease
Vascular Endothelial Growth Factor Receptor-2
biology.protein
Interleukin-23 Subunit p19
Surgery
business
Biomarkers
Psoriasis/pathology
Follow-Up Studies
Zdroj: Lasers in Surgery and Medicine, 42(3), 201-210. Wiley-Liss Inc.
Lasers in Surgery and Medicine, 42(3), 201-210. Wiley
ISSN: 0196-8092
DOI: 10.1002/lsm.20898
Popis: Background and Objectives Pulsed dye laser (PDL) therapy is effective in clearing psoriasis plaques, but the mechanism of action is only partially understood. Local narrow-band ultraviolet B (NB-UVB), which has a better-defined mode of action, is an effective standard treatment for psoriasis. Our aim was to evaluate the cellular and molecular effects of PDL and to compare them with those of local NB-UVB in order to gain further insight into their mechanisms of action in psoriasis. Study Design/Patients and Methods Nineteen patients with stable plaque-type psoriasis were treated either with PDL or NB-UVB. Lesional punch biopsies were obtained from all patients before treatment. Additional biopsies were obtained at 3 and 24 hours after PDL treatment in five of these patients. In 14 patients additional biopsies were taken after 7 and 13 weeks of treatment. Samples were histopathologically examined for the level of dermal T cell infiltrate, and the expression of epidermal β-defensin 2, immune cell-derived tumor necrosis factor (TNF)-α, endothelial E-selectin, vascular endothelial growth factor receptor (VEGFR) 2 and 3, and the expression of interleukin (IL)-23 before and after treatment. Results The expression of VEGFR2, VEGFR3, and E-selectin was decreased in clinically high responders within 24 hours after PDL treatment. The expression of IL-23, TNF-α mRNA, and E-selectin protein were significantly reduced after two PDL treatments, whereas the expression of all epidermal markers and dermal T cell infiltrates had normalized after four treatments. The expression of epidermal activation markers and E-selectin were significantly reduced after 13 weeks of NB-UVB treatment. Conclusions The expression of epidermal activation markers and the dermal T cell infiltrates were decreased after both treatments. The decreased expression of VEGFR2 and VEGFR3 followed by the down-regulation of TNF-α and IL-23p19 may be contributory factors in the efficacy of PDL in stable plaque-type psoriasis. Lasers Surg. Med. 42:201–210, 2010. © 2010 Wiley-Liss, Inc.
Databáze: OpenAIRE
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