Activation of Signal Pathways of Apoptosis under Conditions of Prolonged ER-Stress Caused by Exposure of Mouse Testicular Teratoma Cells to Selenium-Containing Compounds
| Autor: | E E Fesenko, M V Goltyaev, E G Varlamova, S V Novoselov |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Mouse Testicular Teratoma Cell Survival Biophysics Apoptosis medicine.disease_cause Biochemistry 03 medical and health sciences Mice Necrosis Testicular Neoplasms Cell Line Tumor Organoselenium Compounds medicine Animals MTT assay Phosphorylation Selenium Compounds 030304 developmental biology 0303 health sciences Chemistry Endoplasmic reticulum 030302 biochemistry & molecular biology Teratoma General Chemistry General Medicine Endoplasmic Reticulum Stress Molecular biology Blot Oxidative Stress Microscopy Fluorescence Unfolded protein response Signal transduction Drug Screening Assays Antitumor Carcinogenesis Signal Transduction |
| Zdroj: | Doklady. Biochemistry and biophysics. 490(1) |
| ISSN: | 1608-3091 |
| Popis: | Aim to study the molecular mechanisms of apoptotic death of mouse testicular teratocarcinoma cells (line F-9) under exposure to the widely used selenium-containing compounds with antitumor activity, sodium selenite and methylseleninic acid. Methods fluorescence microscopy, MTT assay, Western blotting. Results It was shown that sodium selenite at a concentration of 10 μM and methylseleninic acid at concentrations of 1 and 10 μM cause apoptosis-dependent death of F-9 cells, excluding necrotic death. Western blotting showed an increase in the expression of XBP1s when treating F-9 cells with 1 μM methylseleninic acid. Conclusions 10 μM methylseleninic acid leads to cell apoptosis, most likely by activation of the IRE1 signaling pathway under prolonged stress of the endoplasmic reticulum. |
| Databáze: | OpenAIRE |
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