Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
| Autor: | Juergen C Jung, Fan Shi, Hannah E. Yamamoto, Daniel W. Awari, Masayuki Yazawa, Seon-Hye E. Park, Jose R. Quejada, Fuun Kawano |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Transcriptional Activation 0301 basic medicine Light Kelch Repeat Arabidopsis Flavin group Biology 3T3 cells Cell Line Mice 03 medical and health sciences 0302 clinical medicine Transcription (biology) Basic Helix-Loop-Helix Transcription Factors Genetics medicine Animals Humans Arabidopsis Proteins HEK 293 cells Gigantea 3T3 Cells biology.organism_classification 3. Good health Cell biology Cryptochromes Mice Inbred C57BL Optogenetics HEK293 Cells 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Cell culture Methods Online Female sense organs 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkx804 |
| Popis: | Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (GI) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells. |
| Databáze: | OpenAIRE |
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