Inoculation of Lewis lung carcinoma cells enhances formalin-induced pain behavior and spinal Fos expression in mice
| Autor: | Jin Bong Park, Yeonhee Ryu, Jae-Min Kim, Hyun-Woo Kim, Jung-Wan Choi, Seong-Hun Ahn, Dong-Wook Kang, Jae-Gyun Choi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Pathology medicine.medical_specialty Spinal Cord Dorsal Horn Cell Pain Ibuprofen lung neoplasms 03 medical and health sciences Carcinoma Lewis Lung Mice 0302 clinical medicine Formaldehyde medicine Animals Lung cancer General Veterinary business.industry Inoculation Fos Lewis lung carcinoma Cancer Cancer Pain Analgesics Non-Narcotic medicine.disease Pathophysiology formalin Mice Inbred C57BL medicine.anatomical_structure Oncogene Proteins v-fos Spinal Cord 030220 oncology & carcinogenesis Cancer cell Immunology Original Article business 030217 neurology & neurosurgery Neoplasm Transplantation medicine.drug |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| Popis: | The incidence of lung cancer has rapidly increased and cancer patients at a later cancer stage frequently suffer from unbearable cancer-associated pain. However, the pathophysiology of lung cancer pain has not been fully described due to a lack of appropriate animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cells (1.5 × 105, 2.5 × 105, 3.0 × 105 or 5.0 × 105) were inoculated into back or peri-sciatic nerve areas. Back area inoculation was adopted to determine the effect of cancer cell circulating factors and the peri-sciatic nerve area was used to evaluate the possible effects of cancer cell contacting and circulating factors on formalin-induced pain. At postinoculation day 7, LLC cell (5.0 × 105) inoculations in both back and peri-sciatic nerve area significantly increased formalin-induced paw-licking time and spinal Fos expression over those in cell-media-inoculated (control) mice. Enhanced pain behavior and spinal Fos expression were significantly suppressed by ibuprofen pretreatment (250 mg/kg). The results of this study suggest that LLC cell circulating factors and inflammatory responses may be critical in enhancing pain sensation in the early stage of lung cancer cell inoculation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|