Production of an acetone-butanol-ethanol mixture from Clostridium acetobutylicum and its conversion to high-value biofuels
| Autor: | Adam Grippo, Sanil Sreekumar, Douglas S. Clark, Gorkem Gunbas, Anbarasan Pazhamalai, Harvey W. Blanch, F. Dean Toste, Zachary C. Baer |
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| Rok vydání: | 2015 |
| Předmět: |
Clostridium acetobutylicum
Magnesium Hydroxide Butanols Aluminum Hydroxide General Biochemistry Genetics and Molecular Biology law.invention Acetone chemistry.chemical_compound Bioreactors law Bioreactor Distillation Ethanol Chromatography biology business.industry Butanol food and beverages Ketones equipment and supplies biology.organism_classification Biotechnology Biosynthetic Pathways chemistry Biofuel Biofuels Fermentation business Palladium |
| Zdroj: | Nature protocols. 10(3) |
| ISSN: | 1750-2799 |
| Popis: | Clostridium acetobutylicum is a bacterial species that ferments sugar to a mixture of organic solvents (acetone, butanol and ethanol). This protocol delineates a methodology to combine solventogenic clostridial fermentation and chemical catalysis via extractive fermentation for the production of biofuel blendstocks. Extractive fermentation of C. acetobutylicum is operated in fed-batch mode with a concentrated feed solution (500 grams per liter glucose and 50 grams per liter yeast extract) for 60 h, producing in excess of 40 g of solvents (acetone, butanol and ethanol) between the completely immiscible extractant and aqueous phases of the bioreactor. After distillation of the extractant phase, the acetone, butanol and ethanol mixture is upgraded to long-chain ketones over a palladium-hydrotalcite (Pd-HT) catalyst. This reaction is generally carried out in batch with a high-pressure Q-tube for 20 h at 250 °C. Following this protocol enables the production of ∼0.5 g of high-value biofuel precursors from a 1.7-g portion of fermentation solvents. |
| Databáze: | OpenAIRE |
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