Identification of miRNAs Enriched in Extracellular Vesicles Derived from Serum Samples of Breast Cancer Patients
| Autor: | Daniela Fiori Gradia, Thalita Regina Tuleski, Emanuel Maltempi de Souza, Silvio M. Zanata, Cicero Urban, Vânia C. S. Pankievicz, Ingrid Larissa Melo de Souza, Patricia Midori Murobushi Ozawa, Pryscilla Fanini Wowk, Enilze Maria de Souza Fonseca Ribeiro, Débora S. Lemos, Danielle Malheiros, Evelyn Vieira, Iglenir J. Cavalli, Luciane R. Cavalli, Rubens Silveira de Lima, Rodrigo Coutinho de Almeida, Flavia Kuroda |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty lcsh:QR1-502 Breast Neoplasms Triple Negative Breast Neoplasms Biochemistry Extracellular vesicles Article lcsh:Microbiology 03 medical and health sciences 0302 clinical medicine Breast cancer microRNA Biomarkers Tumor medicine Humans Liquid biopsy Molecular Biology Aged miRNA liquid biopsy business.industry Gene Expression Profiling Area under the curve Cancer RNA Middle Aged medicine.disease Gene Expression Regulation Neoplastic MicroRNAs Circulating MicroRNA 030104 developmental biology 030220 oncology & carcinogenesis circulating microRNAs Female RNA-seq business extracellular vesicles |
| Zdroj: | Biomolecules Volume 10 Issue 1 Biomolecules, Vol 10, Iss 1, p 150 (2020) |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom10010150 |
| Popis: | MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis as a screening strategy to identify EV-miRNAs derived from serum of clinically well-annotated breast cancer (BC) patients from the south of Brazil. EVs from three groups of samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated from serum using a precipitation method and analyzed by RNA-seq (screening phase). Subsequently, four EV-miRNAs (miR-142-5p, miR-150-5p, miR-320a, and miR-4433b-5p) were selected to be quantified by quantitative real-time PCR (RT-qPCR) in individual samples (test phase). A panel composed of miR-142-5p, miR-320a, and miR-4433b-5p distinguished BC patients from CT with an area under the curve (AUC) of 0.8387 (93.33% sensitivity, 68.75% specificity). The combination of miR-142-5p and miR-320a distinguished LA patients from CT with an AUC of 0.9410 (100% sensitivity, 93.80% specificity). Interestingly, decreased expression of miR-142-5p and miR-150-5p were significantly associated with more advanced tumor grades (grade III), while the decreased expression of miR-142-5p and miR-320a was associated with a larger tumor size. These results provide insights into the potential application of EVs-miRNAs from serum as novel specific markers for early diagnosis of BC. |
| Databáze: | OpenAIRE |
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