Estimation by an electrophysiological method of the expression of oxytocin receptor mRNA in human myometrium during pregnancy
| Autor: | Tadashi Kimura, Osamu Tanizawa, M. Ono, Masahiko Takemura, Yoshihiro Tokugawa, Chihiro Azuma, Fumitaka Saji, Kensaku Mori, Mariko Miki |
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| Rok vydání: | 1992 |
| Předmět: |
endocrine system
medicine.medical_specialty Vasopressin Endocrinology Diabetes and Metabolism Receptor expression Clinical Biochemistry Uterus Gene Expression Biology Biochemistry Oxytocin Antagonist Xenopus laevis Endocrinology Pregnancy Internal medicine medicine Animals Humans RNA Messenger Molecular Biology Microinjection Receptors Angiotensin Electric Conductivity Myometrium Cell Biology Oxytocin receptor Electrophysiology medicine.anatomical_structure Oxytocin Receptors Oxytocin Oocytes Molecular Medicine Biological Assay Female hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 42:253-258 |
| ISSN: | 0960-0760 |
| Popis: | In order to evaluate the changes in uterine oxytocin receptor-specific mRNA during pregnancy, receptor expression in Xenopus oocytes are examined electrophysiologically following microinjection of mRNA from human uterus. In voltage-clamped oocytes injected with term myometrial mRNA, oxytocin elicited an inward current response. The amplitude of the oxytocin-induced current increased with increasing dose of oxytocin, but no current was elicited following stimulation with vasopressin. The oxytocin-induced current was completely eliminated as a result of pretreatment with a specific oxytocin antagonist. 21 of 27 oocytes injected with term myometrial mRNA showed a large amplitude (77.0 +/- 16.1 nA) reaction to oxytocin. In comparison, only 3 of 13 oocytes injected with early gestational myometrial mRNA exhibited a small amplitude (4.6 +/- 1.4 nA) reaction to oxytocin. No oxytocin response was observed in oocytes injected with non-pregnant myometrial mRNA. These results indicate that the striking increment in oxytocin sensitivity in term uterus depends on the increase in mRNA encoding oxytocin receptors. |
| Databáze: | OpenAIRE |
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