Influence of hCG on inducible nitric oxide synthase gene expression in ram testicular arteries
| Autor: | Sonia Marrocchella, Annalisa Rizzo, Ugo Indraccolo, Pantaleo Greco, Elvira Grandone, Giuseppe Cardo, Donatella Colaizzo, Ettore Cicinelli, Maria Matteo, Raffaele Luigi Sciorsci, Laura Castellana, G. Minoia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
endocrine system Intratesticular blood flow Urology hCG Socio-culturale Vasodilation lcsh:RC870-923 Testicular artery Nitric oxide Basal (phylogenetics) chemistry.chemical_compound Internal medicine medicine.artery Gene expression medicine INOS gene INOS gene Testicular artery Ram biology business.industry urogenital system lcsh:Diseases of the genitourinary system. Urology Nitric oxide synthase Endocrinology Real-time polymerase chain reaction chemistry biology.protein Ligation business iNOS gene Testicular artery |
| Zdroj: | Archivio Italiano di Urologia e Andrologia, Vol 86, Iss 3, Pp 183-187 (2014) |
| ISSN: | 2282-4197 1124-3562 |
| Popis: | Background. Experimental evidence suggests a relationship between the vasodilatory effect of hCG and the NOS system in the testis. The influence of hCG administration on testicular vascular NOS gene expression has not been fully investigated. Objective: This study aimed to evaluate the presence of the nitric oxide syntheses gene in ram testicular arteries and the influence of hCG administration on its expression. Materials and methods: Both testicular arteries of sixteen rams were extracted before and after i.v. administration of 5000 IU of hCG or placebo. The expression of the iNOS gene was investigated by real time PCR. Data were analyzed by means of Wilcoxon and Mann-Whitney tests. A p value of < 0.05 was considered statistically significant. Results: PCR revealed the presence of iNOS mRNA in all basal samples but the expression of the iNOS gene was significantly reduced in all arteries obtained 24 h after the administration of either hCG or placebo. A significant reduction in the expression of iNOS gene was observed in the testicular arteries extracted after 24 h in both treated and placebo groups. On the other hand hCG stimulation did not significantly influence iNOS expression following its administration compared to a placebo. Conclusion: Ram testicular arteries express the iNOS gene but hCG stimulation did not significantly influence iNOS expression. A significant reduction in the expression of this gene was observed in the testicular arteries extracted after 24 h in both treated and placebo groups, suggesting that iNOS expression on the testicular artery could be influenced by the spermatic vessel ligation of the controlateral testis. |
| Databáze: | OpenAIRE |
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