Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients
| Autor: | Miguel Bolonio, Gemma Castillejo, Antonio Millán, Victoria Alejandra Jiménez-García, Luis Fernández-Salazar, Alfonso Rodríguez-Herrera, José Carlos Salazar, Carmen Ribes-Koninckx, Eduardo Arranz, Luis Vaquero, Carolina Sousa, Alba Muñoz-Suano, Mercè Rosinach, Justo Valverde, M.A. Montes-Cano, Santiago Vivas, Blanca Fambuena, Ana María Vegas, Luis Ortigosa, Verónica Segura, Alejandro Nuñez, Jorge Marinich, Francesc Martínez Cerezo, Beatriz Espín, Carlos Sierra, Oreste Lo Iacono, Francisco Leon, José Ramón Alberto, César Guajardo, Ángel Caunedo, Francisco José Girón, José Antonio Garrote, Laura Crespo, Ana Galera, Angel Cebolla, Fernando Fernández-Bañares, Maria Esteve, Manuel Romero-Gómez, J M Marugán-Miguelsanz, Isabel Comino |
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| Přispěvatelé: | European Commission, Corporación Tecnológica de Andalucía, Ministerio de Ciencia e Innovación (España), Universidad de Sevilla. Departamento de Microbiología y Parasitología, [Comino,I, Segura,V, Sousa,C] Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain. [Fernández-Bañares,F, Esteve,M, Rosinach,M] Department of Gastroenterology, Hospital Universitari Mutua Terrassa, and CIBERehd, Terrassa, Barcelona, Spain. [Ortigosa,L, Guajardo,C, Alberto, JR] Pediatric Gastroenterology, Hospital Universitario Nuestra Señora de La Candelaria, Tenerife, Spain. [Castillejo,G, Martínez Cerezo,F] Pediatric Gastroenterology, Hospital Universitari de Sant Joan de Reus, IISPV, URV, Reus, Spain. [Fambuena,B, Romero-Gómez,M, Millán,A] Unit for the Clinical Management of Digestive Diseases and CIBERehd and Gastroenterology and Nutrition Unit, Hospital Universitario Virgen de Valme, Seville, Spain. [Ribes-Koninckx,C, Bolonio,M] Pediatric Gastroenterology, Hepatology and Nutrition Unit, Hospital Universitario y Politécnico La Fe, Celiac Disease and Digestive Inmunopatology Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. [Sierra,C, Girón,.FJ] Pediatric Gastroenterology and Nutrition Unit, Hospital Materno-Infantil, Malaga, Spain. [Rodríguez-Herrera,A, Galera,A, Valverde,J] Gastroenterology and Nutrition Unit, Instituto Hispalense de Pediatría, Seville, Spain. [Salazar,JC, Espín,B] Servicio de Gastroenterología Pediátrica, Hospital Universitario Virgen del Rocío, Seville, Spain. [Caunedo,A, Jiménez-García,VA] Hospital Universitario Virgen Macarena, Seville, Spain. [Marugán-MiguelSanz,JM, Fernández-Salazar,L, Arranz,E] Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, CSIC and Gastroenterology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain. [Garrote,JA, Vegas, AM, Crespo,L] Clinical Analysis and Pediatrics, Hospital Universitario Río Hortega, Valladolid, Spain. [Vivas,S, Nuñez,A, Vaquero,LServicio de Aparato Digestivo, Hospital Universitario de Leon, Leon, Spain. [Lo Iacono,O] Sección de Aparato Digestivo, Hospital del Tajo, Madrid, Spain. [Montes-Cano,MA] Servicio de Inmunología, CIBER de Epidemiología y Salud Pública, Hospital Universitario Virgen del Rocío/IBiS/CSIC/Universidad de Sevilla, Seville, Spain. [León,F] Celimmune, Bethesda, Maryland, USA. [Marinich,J, Muñoz-Suano,A, Cebolla,A] Biomedal SL, Seville, Spain., Th is work was supported by grants from Ministerio de Ciencia e Innovación and FEDER funds (DELIAC, IPT-2011-0952-900000), and Corporación Tecnológica de Andalucía (SINGLUCHECK, 1737/0118). |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Proteínas de unión al GTP
Male Pathology Enfermedad celíaca Tissue transglutaminase Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Malabsorption Syndromes::Celiac Disease [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunohistochemistry::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay [Medical Subject Headings] Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens::Gliadin [Medical Subject Headings] Gastroenterology Gliadin Serology Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Feces 0302 clinical medicine Surveys and Questionnaires Estudios prospectivos Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Carrier Proteins::GTP-Binding Proteins [Medical Subject Headings] Ingestion Ensayo de inmunoadsorción enzimática 030212 general & internal medicine Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Surveys and Questionnaires [Medical Subject Headings] Prospective Studies Young adult Prospective cohort study Masculino Child chemistry.chemical_classification biology Health Care::Health Care Quality Access and Evaluation::Delivery of Health Care::Attitude to Health::Patient Acceptance of Health Care::Patient Compliance [Medical Subject Headings] Gliadina Age Factors Diet Records Humanos Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens [Medical Subject Headings] Encuestas y cuestionarios Child Preschool Health Care::Health Care Quality Access and Evaluation::Quality of Health Care::Health Care Evaluation Mechanisms::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Prospective Studies [Medical Subject Headings] 030211 gastroenterology & hepatology Female Inmunoglobulina A medicine.medical_specialty Adolescent Glutens Check Tags::Male [Medical Subject Headings] Enzyme-Linked Immunosorbent Assay Antibodies 03 medical and health sciences Diet Gluten-Free Young Adult GTP-Binding Proteins Internal medicine medicine Chemicals and Drugs::Amino Acids Peptides and Proteins::Peptides [Medical Subject Headings] Humans Protein Glutamine gamma Glutamyltransferase 2 Serologic Tests Cooperación del paciente Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin A [Medical Subject Headings] Autoantibodies Transglutaminases Hepatology business.industry Case-control study Péptidos nutritional and metabolic diseases Infant Dieta sin gluten Glútenes Colon/Small Bowel Gluten Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Aminoacyltransferases::Transglutaminases [Medical Subject Headings] Transglutaminasas digestive system diseases Immunoglobulin A Celiac Disease chemistry Case-Control Studies biology.protein Patient Compliance Gluten free Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet Gluten-Free [Medical Subject Headings] business 3206 Ciencias de la Nutrición |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname UVaDOC: Repositorio Documental de la Universidad de Valladolid Universidad de Valladolid Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid The American Journal of Gastroenterology UVaDOC. Repositorio Documental de la Universidad de Valladolid idUS. Depósito de Investigación de la Universidad de Sevilla AMERICAN JOURNAL OF GASTROENTEROLOGY r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe |
| ISSN: | 0271-1397 2011-0952 0002-9270 |
| Popis: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License.-- et al. [Objectives]: Treatment for celiac disease (CD) is a lifelong strict gluten-free diet (GFD). Patients should be followed-up with dietary interviews and serology as CD markers to ensure adherence to the diet. However, none of these methods offer an accurate measure of dietary compliance. Our aim was to evaluate the measurement of gluten immunogenic peptides (GIP) in stools as a marker of GFD adherence in CD patients and compare it with traditional methods of GFD monitoring. [Methods]: We performed a prospective, nonrandomized, multicenter study including 188 CD patients on GFD and 84 healthy controls. Subjects were given a dietary questionnaire and fecal GIP quantified by enzyme-linked immunosorbent assay (ELISA). Serological anti-tissue transglutaminase (anti-tTG) IgA and anti-deamidated gliadin peptide (anti-DGP) IgA antibodies were measured simultaneously. [Results]: Of the 188 celiac patients, 56 (29.8%) had detectable GIP levels in stools. There was significant association between age and GIP in stools that revealed increasing dietary transgressions with advancing age (39.2% in subjects ≥13 years old) and with gender in certain age groups (60% in men ≥13 years old). No association was found between fecal GIP and dietary questionnaire or anti-tTG antibodies. However, association was detected between GIP and anti-DGP antibodies, although 46 of the 53 GIP stool-positive patients were negative for anti-DGP. [Conclusions]: Detection of gluten peptides in stools reveals limitations of traditional methods for monitoring GFD in celiac patients. The GIP ELISA enables direct and quantitative assessment of gluten exposure early after ingestion and could aid in the diagnosis and clinical management of nonresponsive CD and refractory CD. Trial registration number NCT02711397. This work was supported by grants from Ministerio de Ciencia e Innovación and FEDER funds (DELIAC, IPT-2011-0952-900000), and Corporación Tecnológica de Andalucía (SINGLUCHECK, 1737/0118). |
| Databáze: | OpenAIRE |
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