Bioactive compounds from regular diet and faecal microbial metabolites

Autor: Nuria Salazar, Miguel Gueimonde, Sonia González, Tania Fernández-Navarro, Abelardo Margolles, Borja Sánchez, Patricia Ruas-Madiedo, de Los Reyes-Gavilán Cg, Isabel Gutiérrez-Díaz
Přispěvatelé: Ministerio de Economía y Competitividad (España), Principado de Asturias, European Commission, Ministerio de Ciencia e Innovación (España), Salazar, Nuria, Sánchez García, Borja, Ruas-Madiedo, Patricia, González de los Reyes-Gavilán, Clara, Margolles Barros, Abelardo, Gueimonde Fernández, Miguel, Salazar, Nuria [0000-0003-1435-7628], Sánchez García, Borja [0000-0003-1408-8018], Ruas-Madiedo, Patricia [0000-0001-6158-9320], González de los Reyes-Gavilán, Clara [0000-0001-9396-631], Margolles Barros, Abelardo [0000-0003-2278-1816], Gueimonde Fernández, Miguel [0000-0002-0192-901X]
Rok vydání: 2016
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1436-6215
Popis: [Purpose] Short-chain fatty acids (SCFAs) formation by intestinal bacteria is regulated by many different factors, among which dietary fibre is currently receiving most attention. However, since fibre-rich foods are usually good dietary sources of phenolic compounds, which are also known to affect the microbiota, authors hypothesize that the regular intake of these bioactive compounds could be associated with a modulation of faecal SCFA production by the intestinal microbiota. [Methods] In this work, food intake was recorded by means of a validated Food Frequency Questionnaire. Fibres were determined using Marlett food composition tables, and phenolic compounds were obtained from Phenol-Explorer Database. Analysis of SCFA was performed by gas chromatography–flame ionization/mass spectrometry and quantification of microbial populations in faeces by quantitative PCR. [Results] Klason lignin and its food contributors, as predictors of faecal butyrate production, were directly associated with Bacteroides and Bifidobacterium levels, as well as lignans with Bacteroides. Also, anthocyanidins, provided by strawberries, were associated with faecal propionate and inversely related to Lactobacillus group. [Conclusions] These results support the hypothesis we put forward regarding the association between some vegetable foods (strawberries, pasta, lentils, lettuce and olive oil) and faecal SCFA. More studies are needed in order to elucidate whether these associations have been mediated by the bacterial modulatory effect of the bioactive compounds, anthocyanins, lignans or Klason lignin, present in foodstuffs.
This study was financed by the Spanish Plan Nacional de I+D (Grant AGL2010-14952) and by Biópolis SL. within the framework of the e-CENIT Project SENIFOOD from the Spanish Ministry of Science and Innovation. The activity of the group is being partly supported through the Grant GRUPIN14-043 from “Plan Regional de Investigación” of Principado de Asturias. Both AGL2010-14952 and GRUPIN14-043 received cofounding from European Union FEDER funds. N. Salazar benefits from a “Clarín” postdoctoral contract (Marie Curie European CoFund Program) co-funded by the “Plan Regional de Investigación” of Principado de Asturias, Spain. B. Sánchez was the recipient of a “Ramón y Cajal” postdoctoral contract from the Spanish Ministry of Economy and Competitiveness. The contract of I. Gutierrez-Diaz was supported by grant GRUPIN14-043. We acknowledge the excellent technical assistance of Lidia Alaez, whose contract was partially supported by the Project AGL2010-16525 and by “Plan Regional de Investigación” of Principado de Asturias, through the Grant COF 13-020. We show our greatest gratitude to all the volunteers participating in the study and to “Alimerka Foundation”.
Databáze: OpenAIRE
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