Comparative Genomic Analysis of Classical and Variant Virulent Parental/Attenuated Strains of Porcine Epidemic Diarrhea Virus
| Autor: | Qigai He, Meizhou Wu, Xiaozhen Guo, Fangzhou Chen, Shiyi Ye, Zhonghua Li, Xugang Ku, Yinxing Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
virus attenuation
Swine viruses lcsh:QR1-502 Adaptation Biological Virulence Genome Viral Biology Virus lcsh:Microbiology Article Serial passage Virology Chlorocebus aethiops Animals Serial Passage Gene Vero Cells Sequence Deletion Swine Diseases Viral Structural Proteins Porcine epidemic diarrhea virus virus cell adaptation variant PEDV genomic analysis Genetic Variation Sequence Analysis DNA biology.organism_classification Open reading frame Infectious Diseases Amino Acid Substitution Vero cell Mutant Proteins Synonymous substitution Coronavirus Infections |
| Zdroj: | Viruses Volume 7 Issue 10 Pages 5525-5538 Viruses, Vol 7, Iss 10, Pp 5525-5538 (2015) Viruses; Volume 7; Issue 10; Pages: 5525-5538 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v7102891 |
| Popis: | Since 2010, the variant porcine epidemic diarrhea virus (PEDV) has been the etiological agent responsible for the outbreak of porcine epidemic diarrhea (PED) worldwide. In this study, a variant PEDV strain YN1 was isolated, serially propagated on the Vero cells and was characterized for 200 passages. To better elucidate the molecular basis of Vero cell adaptation of variant PEDV strains, we sequenced, compared, and analyzed the full-genome sequences of parental YN1 and passages 15, 30, 60, 90, 144, and 200. The results showed that the variations increased with the viral passage. The nucleotides sequences of non-structural protein (NSP)2, NSP4-7, NSP10, NSP12 and NSP13 genes did not change during the Vero cell adaptation process. After comparison of the variation characteristic of classical, variant virulent/attenuated strains, it was found that attenuation of PEDV virus was associated with 9-26 amino acid (aa) changes in open reading frames (ORF) 1a/b and S protein, early termination in ORF3, 1–3 aa changes in E, M and N protein and some nucleotide sequences’ synonymous mutations. The aa deletion at about 144 aa of S protein could be the attenuation marker for the PEDV. The pig study showed that the early termination in ORF3 was more important for virus cell adaptation than virus attenuation. |
| Databáze: | OpenAIRE |
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