Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP
| Autor: | Didier Colau, Jean-Christophe Renauld, Laure Dumoutier, Patricia Ribeiro de Moura, Alessandro S. Nascimento, Igor Polikarpov, Leandra Watanabe |
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| Rok vydání: | 2009 |
| Předmět: |
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Molecular Stereochemistry Biophysics Polyethylene glycol Biochemistry law.invention Tetragonal crystal system chemistry.chemical_compound X-Ray Diffraction Structural Biology law Genetics Humans Crystallization Receptor Chemistry Interleukins Binding protein Resolution (electron density) SISTEMA IMUNE Receptors Interleukin Condensed Matter Physics Recombinant Proteins Crystallography Solubility Crystallization Communications X-ray crystallography Decoy Protein Binding |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1744-3091 |
| Popis: | Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22-IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 A resolution. The crystal belonged to the tetragonal space group P4(1), with unit-cell parameters a = b = 67.9, c = 172.5 A, and contained two IL-22-IL-22BP complexes per asymmetric unit. |
| Databáze: | OpenAIRE |
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