Quantification of macrophage content in atherosclerotic plaques by optical coherence tomography
| Autor: | Elkan F. Halpern, H. Thomas Aretz, Milen Shishkov, Kelly Schlendorf, Guillermo J. Tearney, Stuart L. Houser, Christopher R. Kauffman, Hiroshi Yabushita, Brett E. Bouma, Ik-Kyung Jang |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty genetic structures Light Cost effectiveness Arteriosclerosis Antigens Differentiation Myelomonocytic Matrix (biology) Sensitivity and Specificity Muscle Smooth Vascular Optical coherence tomography Antigens CD Physiology (medical) Medicine Humans Tomography Aged Inflammation medicine.diagnostic_test Anatomy Cross-Sectional business.industry CD68 Macrophages Fibrous cap Histology Arteries Actins Staining medicine.anatomical_structure Female Cardiology and Cardiovascular Medicine business |
| Zdroj: | Circulation. 107(1) |
| ISSN: | 1524-4539 |
| Popis: | Background— Macrophage degradation of fibrous cap matrix is an important contributor to atherosclerotic plaque instability. An imaging technology capable of identifying macrophages in patients could provide valuable information for assessing plaque vulnerability. Optical coherence tomography (OCT) is a new intravascular imaging modality that allows cross-sectional imaging of tissue with a resolution of ≈10 μm. The aim of this study was to investigate the use of OCT for identifying macrophages in fibrous caps. Methods and Results— OCT images of 26 lipid-rich atherosclerotic arterial segments obtained at autopsy were correlated with histology. Cap macrophage density was quantified morphometrically by immunoperoxidase staining with CD68 and smooth muscle actin and compared with the standard deviation of the OCT signal intensity at corresponding locations. There was a high degree of positive correlation between OCT and histological measurements of fibrous cap macrophage density ( r =0.84, P r =−0.56, P 10% CD68 staining. Conclusions— The high contrast and resolution of OCT enables the quantification of macrophages within fibrous caps. The unique capabilities of OCT for fibrous cap characterization suggest that this technology may be well suited for identifying vulnerable plaques in patients. |
| Databáze: | OpenAIRE |
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