Prototype single step lateral flow technology for detection of avian influenza virus and chicken antibody to avian influenza virus
| Autor: | E. G. Brown, Klaus Nielsen, Jamshid Tanha, R. MacKenzie, W L Yu, C. Elmgren, L. Keleta, M. Lin, S. A. Nadin Davis, G. Dubuc, Shenghua Li, John Pasick |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.drug_class
Clinical Biochemistry Immunology Gold Colloid Monoclonal antibody Antibodies Viral Sensitivity and Specificity Epitope Virus law.invention Antigen law medicine Immunology and Allergy Animals Antigens Viral Latex beads Immunoassay biology Antibodies Monoclonal Molecular biology Microspheres Recombinant Proteins Medical Laboratory Technology Colloidal gold Influenza A virus Influenza Vaccines Influenza in Birds biology.protein Recombinant DNA Lateral flow technology Avian influenza virus Chicken antibody Antibody Chickens |
| Zdroj: | Journal of immunoassayimmunochemistry. 28(4) |
| ISSN: | 1532-1819 |
| Popis: | A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti‐chicken antibody was attached, were used as the indicator system. |
| Databáze: | OpenAIRE |
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