The Expression of SPARC in Adipose Tissue and Its Increased Plasma Concentration in Patients with Coronary Artery Disease
| Autor: | Hiroshi Kuriyama, Kikuko Hotta, Ken Kishida, Yuji Matsuzawa, Hitoshi Nishizawa, Shizuya Yamashita, Tohru Funahashi, Iichiro Shimomura, Noriyuki Ouchi, Kazuhisa Maeda, Masahiko Takahashi, Norikazu Maeda, Hiroyuki Nagaretani, Tadashi Nakamura, Shinji Kihara |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Gene Expression Medicine (miscellaneous) Adipose tissue Coronary Disease Body Mass Index Immunoenzyme Techniques Coronary artery disease Mice chemistry.chemical_compound Endocrinology In vivo Internal medicine Adipocyte RNA Ribosomal 28S Gene expression Animals Humans Medicine Osteonectin Obesity RNA Messenger Northern blot biology business.industry Public Health Environmental and Occupational Health Middle Aged Blotting Northern medicine.disease Up-Regulation Mice Inbred C57BL Blot Adipose Tissue chemistry biology.protein Female business Food Science |
| Zdroj: | Obesity Research. 9:388-393 |
| ISSN: | 1071-7323 |
| DOI: | 10.1038/oby.2001.50 |
| Popis: | Objective: Adipocytes secrete various cytokines and matrix proteins. Several of them precipitate in obesity-associated diseases, including atherosclerosis. In the current study, we have examined the expression of secreted protein, acidic and rich in cysteine (SPARC) in adipose tissue and its significance in obesity and coronary artery disease (CAD). Research Methods and Procedures: The SPARC mRNA expressions both in vivo and in vitro were detected by Northern blot analysis. Plasma SPARC concentrations were measured by enzyme immunosorbent assay. First, we investigated the plasma SPARC levels of 88 unrelated adult Japanese subjects (62 men and 26 women; average age: [± SD] 50 ± 12 years; body mass index [BMI]: 16 to 46 kg/m2). Additionally 31 subjects with CAD diagnosed by coronary angiography (20 men and 11 women) were also investigated. Results: Human adipose tissues expressed abundant SPARC mRNA. SPARC expression in adipose tissues was upregulated in obese db/db mice. Markedly enhanced expression of SPARC mRNA was observed in 3T3-L1 fibroblasts during adipocyte differentiation. Consistent with these results, plasma SPARC levels proved a positive correlation with BMI in humans (r = 0.27; p < 0.01). Interestingly, plasma SPARC concentrations were significantly elevated in age- and BMI-matched subjects with CAD (p < 0.05). Discussion: SPARC was expressed in adipose tissues and its expression was enhanced in obese mice. In human, plasma SPARC levels were elevated in obesity and CAD patients. This elevated SPARC may be involved in the progression of CAD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text