Antiproliferative effects of celecoxib in Hep-2 cells through telomerase inhibition and induction of apoptosis
| Autor: | Xue-Mei Chen, Hai-Ling Zhang, Yong-Qiang Zhao, Hui Zhang, Xian-Liang Fan, Tao Jia, Hui-Wei Feng, An-Ting Xu |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Telomerase Epidemiology Blotting Western Apoptosis Real-Time Polymerase Chain Reaction Immunoenzyme Techniques chemistry.chemical_compound Tumor Cells Cultured Humans MTT assay Telomerase reverse transcriptase RNA Messenger Laryngeal Neoplasms Cell Proliferation Sulfonamides TUNEL assay Cyclooxygenase 2 Inhibitors Reverse Transcriptase Polymerase Chain Reaction Public Health Environmental and Occupational Health Flow Cytometry Molecular biology Squamous carcinoma Oncology chemistry Cell culture Celecoxib Cancer research Carcinoma Squamous Cell Pyrazoles Growth inhibition |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 15(12) |
| ISSN: | 2476-762X |
| Popis: | Background: To investigate the effect of celecoxib on telomerase activity and apoptosis in a human laryngeal squamous carcinoma cell line (Hep-2 cells). Materials and Methods: The growth inhibition rate of Hep-2 cells in vitro was measured by MTT assay, and apoptosis by TUNEL assay and flow cytometry (FCM). The TRAP-ELISA method was used to determine telomerase activity in Hep-2 cells. The mRNA expression of human telomerase RNA component(hTR), human telomerase reverse transcriptase (hTERT) and human telomerase-associated protein(hTEP1) was determined by RT-PCR assay. Expression of Bax and Bcl-2 proteins was assessed by Western blotting. Results: Celecoxib can inhibit proliferation and induce apoptosis in a dose- and time-dependent manner, repress telomerase activity, decrease hTERT mRNA and Bcl-2 protein expression and increase Bax protein expression, PGE2 had no effect on telomerase. Conclusions: Celecoxib had the antiproliferative and pro-apoptotic effect in Hep-2 cells. Apoptosis was accompanied by a decrease in telomerase activity which was directly correlated with hTERT mRNA and up-regulation of Bax/Bcl-2. Bcl-2 may thus play an important role in telomerase activity as well as apoptosis. |
| Databáze: | OpenAIRE |
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