Ultra-small solid archaeolipid nanoparticles for active targeting to macrophages of the inflamed mucosa
| Autor: | Maria Jose Morilla, Rodrigo Villares Portugal, Leticia Herminia Higa, Eder Lilia Romero, Horacio Emanuel Jerez, Marcelo Alexandre de Farias |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Anti-Inflammatory Agents Medicine (miscellaneous) Nanoparticle DIGESTION STABILITY 02 engineering and technology Dexamethasone Mice chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine polycyclic compounds General Materials Science Otras Nanotecnología Halorubrum Intestinal Mucosa 021001 nanoscience & nanotechnology Interleukin-12 Lipids Biochemistry 030220 oncology & carcinogenesis 0210 nano-technology Digestion hormones hormone substitutes and hormone antagonists medicine.drug endocrine system Materials science Biomedical Engineering Bioengineering INGENIERÍAS Y TECNOLOGÍAS Development Cell Line 03 medical and health sciences Phosphatidylcholine medicine Animals Humans Inflammation Nanotecnología ORAL DELIVERY Interleukin-6 Tumor Necrosis Factor-alpha Macrophages INFLAMMATORY BOWEL DISEASE In vitro digestion Molecular biology In vitro Halorubrum tebenquichense chemistry Nanoparticles Caco-2 Cells |
| Zdroj: | Nanomedicine. 12:1165-1175 |
| ISSN: | 1748-6963 1743-5889 |
| DOI: | 10.2217/nnm-2016-0437 |
| Popis: | Aim: Develop nanoparticulate agents for oral targeted delivery of dexamethasone (Dex) to macrophages of inflamed mucosa. Materials & methods: Solid archaeolipid nanoparticles (SAN-Dex) (compritol/Halorubrum tebenquichense polar archaeolipids/soybean phosphatidylcholine/Tween-80 4; 0.9; 0.3; 3% w/w) loaded with Dex were prepared. Their mucopenetration, stability under digestion and in vitro anti-inflammatory activity, were determined. Results: Ultra-small SAN-Dex strongly reduced the levels of TNF-α, IL-6 and IL-12 on J774A1 cells stimulated with lipopolysaccharides as compared with free Dex or loaded in ordinary solid lipid nanoparticles-Dex. After in vitro digestion, the anti-inflammatory activity of SAN-Dex was retained, while that of solid lipid nanoparticles-Dex was lost. Conclusion: Because of their structural and pharmacodynamic features, SAN-Dex may be suitable for oral targeted delivery to inflamed mucosa. Fil: Higa, Leticia Herminia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jerez, Horacio Emanuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: de Farias, Marcelo Alexandre. Brazilian Nanotechnology National Laboratory; Brasil Fil: Portugal, Rodrigo Villares. Brazilian Nanotechnology National Laboratory; Brasil Fil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina Fil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; Argentina |
| Databáze: | OpenAIRE |
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