| Popis: |
The polymorphic human DNA sequence in the promoter region of the dopamine D4 receptor gene has been investigated by means of non-invasive DNA sampling, amplification by means of the polymerase chain reaction, and subsequent separation of the reaction products by horizontal slab-gel electrophoresis. The single nucleotide polymorphism at the −521 st position upstream of the initiation codon of the dopamine D4 receptor gene has two variations in the human population, a −521 C form and a −521 T form. Genotyping of −521 C/T single nucleotide polymorphism was performed by two independent methods — use of bidirectional allele-specific amplification, as described recently, and by restriction fragment site polymorphism using Fsp I enzyme. Only results giving the same genotypes by both methods were accepted. The genotyping methods were used to compare allele and genotype frequencies in 47 drug-user males and 118 controls. Our preliminary results showed a tendency for a higher frequency of the −521 C allele among dr... |
