An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces
| Autor: | L. Valíček, B. Šmíd, Zora Nevorankova, L. Rodák |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Diarrhea
Swine Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Microbiology Article Virus Immunoenzyme Techniques Viral Matrix Proteins Feces Nidovirales Rotavirus medicine Animals Coronaviridae Porcine epidemic diarrhoea virus (PEDV) Antibodies Blocking Coronavirus Swine Diseases General Veterinary biology Antibodies Monoclonal General Medicine biology.organism_classification Virology Microscopy Electron biology.protein Electrophoresis Polyacrylamide Gel Monoclonal antibodies Antibody Coronavirus Infections Viral gastroenteritis of swine Porcine epidemic diarrhea virus Blocking ELISA |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2004.09.020 |
| Popis: | Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed. |
| Databáze: | OpenAIRE |
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