Site-specific recombinases for manipulation of the mouse genome
| Autor: | Marie-Christine Birling, Xavier Warot, Françoise Gofflot |
|---|---|
| Přispěvatelé: | Institut Clinique de la Souris ( ICS ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Peney, Maité, Institut Clinique de la Souris (ICS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
media_common.quotation_subject
MESH : Animals Genetically Modified Mutagenesis (molecular biology technique) Computational biology Biology MESH: Gene Targeting Genome MESH : Genetic Engineering Animals Genetically Modified MESH: Animals Genetically Modified 03 medical and health sciences Mice 0302 clinical medicine MESH : DNA Nucleotidyltransferases MESH : Mice Recombinase Animals MESH: Animals MESH: Genome Function (engineering) Gene MESH: Mice 030304 developmental biology media_common MESH: Mutagenesis Genetics 0303 health sciences Recombinase-mediated cassette exchange MESH: DNA Nucleotidyltransferases Gene targeting food and beverages MESH : Mutagenesis Workflow Mutagenesis DNA Nucleotidyltransferases Gene Targeting MESH: Genetic Engineering MESH : Animals Genetic Engineering MESH : Genome 030217 neurology & neurosurgery MESH : Gene Targeting |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2009, 561, pp.245-63. 〈10.1007/978-1-60327-019-9_16〉 Methods in Molecular Biology, 2009, 561, pp.245-63. ⟨10.1007/978-1-60327-019-9_16⟩ Transgenesis Techniques ISBN: 9781603270182 |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-60327-019-9_16 |
| Popis: | International audience; Site-specific recombination systems are widespread and popular tools for all scientists interested in manipulating the mouse genome. In this chapter, we focus on the use of site-specific recombinases (SSR) to unravel the function of genes of the mouse. In the first part, we review the most commonly used SSR, Cre and Flp, as well as the newly developed systems such as Dre and PhiC31, and we present the inducible SSR systems. As experience has shown that these systems are not as straightforward as expected, particular attention is paid to facts and artefacts associated with their production and applications to study the mouse genome. In the next part of this chapter, we illustrate new applications of SSRs that allow engineering of the mouse genome with more and more precision, including the FLEX and the RMCE strategies. We conclude and suggest a workflow procedure that can be followed when using SSR to create your mouse model of interest. Together, these strategies and procedures provide the basis for a wide variety of studies that will ultimately lead to the analysis of the function of a gene at the cellular level in the mouse. |
| Databáze: | OpenAIRE |
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