| Autor: |
A. Abouelsayed, A.M. Hezma, Gamal S. El-Bahy, Abdelrazek B. Abdelrazzak |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 287:122093 |
| ISSN: |
1386-1425 |
| DOI: |
10.1016/j.saa.2022.122093 |
| Popis: |
In this study, we investigate the abscopal effect induced in the brain, lung and kidney as a result of partial irradiation of experimental animals with 2 Gy γ-rays. Modifications in the protein secondary structure were used as indicator for the abscopal effect. FTIR spectroscopy and analysis of the amide I and amide II absorption bands suggested possible modifications in the protein secondary structure in the brain and kidney following irradiation. Significant shift in the amide I band was recorded only in the brain. However, the amide I/amide II band area ratio for the three organs examined varied differentially in the irradiated groups as compared with the sham-irradiated group. Employing the lorentzian model to analyze the amide I band of the FTIR spectra, we dissected the amide I band into its components, each component represents one form of the protein secondary structure. Calculation of the weight percentage contribution of each of the protein secondary structure revealed decrease in the α-helix contribution associated with equivalent increase in β-sheets and turns/random coils contributions in the brain and kidney, however the response was more evident in the brain. No change in the α-helix or β-sheets contributions was reported in the lung following irradiation. The data suggest the induction of abscopal effect in the brain and kidney rather than the lung in the form of protein conformation modification. The data also indicate that the abscopal effect is comparable to the effect of direct irradiation in both of the brain and kidney. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect