Evaluation of the digestibility of solid lipid nanoparticles of glyceryl dibehenate produced by two techniques: Ultrasonication and spray-flash evaporation
| Autor: | Cédric Miolane, Frédéric Demarne, Vincent Jannin, Lucia Blas, Stéphanie Chevrier, Denis Spitzer |
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| Rok vydání: | 2017 |
| Předmět: |
Sonication
Glyceride Chemistry Pharmaceutical Lipolysis Pharmaceutical Science Excipient 02 engineering and technology 030226 pharmacology & pharmacy Models Biological Excipients 03 medical and health sciences chemistry.chemical_compound Surface-Active Agents 0302 clinical medicine Pulmonary surfactant Solid lipid nanoparticle medicine Technology Pharmaceutical Lipase Particle Size Chromatography biology Fatty Acids 021001 nanoscience & nanotechnology chemistry Pancreatin biology.protein Nanoparticles lipids (amino acids peptides and proteins) Digestion Glyceryl behenate Particle size 0210 nano-technology medicine.drug |
| Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 111 |
| ISSN: | 1879-0720 |
| Popis: | Objective To evaluate the digestibility of Solid Lipid Nanoparticles (SLN) of glyceryl dibehenate prepared either with surfactants by ultrasonication or without surfactant by spray-flash evaporation. Methods SLN of glyceryl dibehenate (Compritol® 888 ATO) were produced by two processes: (i) high-shear homogenization with a solution of water-soluble surfactants followed by ultrasonication (ii) and Spray-Flash Evaporation (SFE) of the pure lipid. The digestibility of these nanoparticles was then tested by in vitro lipolysis using a pH-stat apparatus and the assay of glycerides by gel phase chromatography. Results SLN of glyceryl dibehenate prepared by ultrasonication exhibited a mean particle size of 180 nm and showed a limited digestion of the lipid excipient. SLN comprising only glyceryl dibehenate produced by SFE have a mean particle size between 235 and 411 nm depending on process parameters. These nanoparticles were not digested by lipases. The presence of surfactant at the lipid/water interface of the SLN seems to be mandatory to allow the adsorption of the lipase and degradation of glyceryl behenate. Conclusions Glyceryl dibehenate as a solid particle – even as a SLN – is not digested by pancreatin during in vitro lipolysis test. |
| Databáze: | OpenAIRE |
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