Tumour infiltrating lymphocytes and immune-related genes as predictors of outcome in pancreatic adenocarcinoma

Autor: Deborah Bonazza, Nicolò de Manzini, Stefan Bagby, A. Guglielmi, Alberto D'Angelo, Navid Sobhani, Giandomenico Roviello, Cristina Bottin, Fabrizio Zanconati, Fabiola Giudici, Daniele Generali
Přispěvatelé: D’Angelo, Alberto, Sobhani, Navid, Roviello, Giandomenico, Bagby, Stefan, Bonazza, Deborah, Bottin, Cristina, Giudici, Fabiola, Zanconati, Fabrizio, De Manzini, Nicolo, Guglielmi, Alessandra, Generali, Daniele
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Cancer Treatment
Gene Expression
0302 clinical medicine
Adenocarcinomas
Cellular types
Medicine and Health Sciences
Nanotechnology
521 immune system genes
Multidisciplinary
biology
Immune cells
FOXP3
Middle Aged
Prognosis
medicine.anatomical_structure
Oncology
tumor infiltrating lymphocytes
030220 oncology & carcinogenesis
Medicine
Adenocarcinoma
White blood cells
Engineering and Technology
Female
Research Article
Cell biology
Blood cells
Science
T cell
Immunology
T cells
Cytotoxic T cells
PTPRC
Carcinomas
03 medical and health sciences
Immune system
Lymphocytes
Tumor-Infiltrating
pancreatic adenocarcinoma
Diagnostic Medicine
medicine
Genetics
Humans
B cell
Aged
Biology and life sciences
Tumor-infiltrating lymphocytes
business.industry
Cancers and Neoplasms
medicine.disease
Pancreatic Neoplasms
030104 developmental biology
Animal cells
Immune System
biology.protein
Cancer research
business
tumor infiltrating lymphocyte
Transcriptome
CD8
Zdroj: PLoS ONE
D'Angelo, A, Sobhani, N, Roviello, G, Bagby, S, Bonazza, D, Bottin, C, Giudici, F, Zanconati, F, De Manzini, N, Guglielmi, A & Generali, D 2019, ' Tumour infiltrating lymphocytes and immune-related genes as predictors of outcome in pancreatic adenocarcinoma ', PLoS ONE, vol. 14, no. 8, e0219566 . https://doi.org/10.1371/journal.pone.0219566
PLoS ONE, Vol 14, Iss 8, p e0219566 (2019)
ISSN: 1932-6203
Popis: BackgroundWe investigated the correlation between pancreatic ductal adenocarcinoma patient prognosis and the presence of tumour infiltrating lymphocytes and expression of 521 immune system genes.MethodsIntratumoural CD3+, CD8+, and CD20+ lymphocytes were examined by immunohistochemistry in 12 PDAC patients with different outcomes who underwent pancreaticoduodenectomy. The results were correlated with gene expression profile using the digital multiplexed NanoString nCounter analysis system (NanoString Technologies, Seattle, WA, USA).ResultsTwenty immune system genes were significantly differentially expressed in patients with a good prognosis relative to patients with a worse prognosis: TLR2 and TLR7 (Toll-like receptor superfamily); CD4, CD37, FOXP3, PTPRC (B cell and T cell signalling); IRF5, IRF8, STAT1, TFE3 (transcription factors); ANP32B, CCND3 (cell cycle); BTK (B cell development); TNF, TNFRF1A (TNF superfamily); HCK (leukocyte function); C1QA (complement system); BAX, PNMA1 (apoptosis); IKBKE (NFκB pathway). Differential expression was more than twice log 2 for TLR7, TNF, C1QA, FOXP3, and CD37.DiscussionTumour infiltrating lymphocytes were present at higher levels in samples from patients with better prognosis. Our findings indicate that tumour infiltrating lymphocyte levels and expression level of the immune system genes listed above influence pancreatic ductal adenocarcinoma prognosis. This information could be used to improve selection of best responders to immune inhibitors.
Databáze: OpenAIRE
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