Microcystin-LR retards gonadal maturation through disrupting the growth hormone/insulin-like growth factors system in zebrafish

Autor: Ping Xie, Jie Hou, Yujing Su, Gu Zemao, Jun Chen, Honghui Guo, Li Li, Wang Lin
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Microcystin-LR
010501 environmental sciences
01 natural sciences
chemistry.chemical_compound
Sexual maturity
Enzyme Inhibitors
Insulin-Like Growth Factor I
Zebrafish
media_common
biology
Reproduction
Brain
General Medicine
Spermatozoa
Pollution
Liver
Female
Development of the gonads
endocrine system
medicine.medical_specialty
Microcystins
media_common.quotation_subject
Cyanobacteria
03 medical and health sciences
Somatomedins
Internal medicine
medicine
Animals
Gonads
0105 earth and related environmental sciences
Life Cycle Stages
Insulin
Ovary
Insulin-like growth factor 2 receptor
Public Health
Environmental and Occupational Health
Environmental Exposure
biology.organism_classification
Sperm
030104 developmental biology
Endocrinology
chemistry
Growth Hormone
Marine Toxins
Water Pollutants
Chemical
Zdroj: Ecotoxicology and Environmental Safety. 139:27-35
ISSN: 0147-6513
Popis: Recent studies have documented that microcystins (MCs) have potential toxic effects on growth and reproduction in fish. However, no systematic data exist on whether MCs cause gonadal development retardation through disrupting the growth hormone/insulin-like growth factors (GH/IGFs) system. To this end, zebrafish hatchlings (5 d post-fertilization) were exposed to 0, 0.3, 3 and 30µg/L microcystin-LR (MC-LR) for 90 d until they reached sexual maturity. Life-cycle exposure to MC-LR caused delayed ovarian maturation and sperm development along with ultrapathological lesions in the brain and liver. Moreover, the retarded gonadal development was accompanied by an inhibition of the GH/IGFs system, which was characterized by significant decreases in the transcriptional levels of brain gh (males only), hepatic igf2a and igf2b as well as gonadal igf1 (males only), igf3 and igf2r. These findings for the first time point to the influence of MC-LR on fish gonadal development via the GH/IGFs system. Also, sex-differential impairments suggested that gonadal development of males is more vulnerable than that of female to MC-LR. Our results provide evidence that MC-LR at environmentally relevant concentrations is able to induce impairments on fish gonadal development.
Databáze: OpenAIRE
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