Downregulation of the constitutively expressed Hsc70 in diabetic myocardium is mediated by insulin deficiency
| Autor: | Ruben Mestril, Hong Da Lin, Hou Fen Su, Harn Shen Chen, Shing Jong Lin, Jaw Wen Chen, Ping H. Wang, Jia Ying Yang, Hui Chin Lai, Jia Jia |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty animal structures Phlorizin Endocrinology Diabetes and Metabolism medicine.medical_treatment Genetic Vectors Immunoblotting Down-Regulation macromolecular substances Biology Kidney Adenoviridae Cell Line Diabetes Mellitus Experimental Receptor IGF Type 1 chemistry.chemical_compound Endocrinology Sodium-Glucose Transporter 1 Downregulation and upregulation Transduction Genetic Internal medicine Diabetes mellitus Heat shock protein medicine Animals Insulin HSP70 Heat-Shock Proteins Insulin-Like Growth Factor I Muscle Skeletal Cells Cultured Myocardium Cardiac muscle HSC70 Heat-Shock Proteins Kidney metabolism medicine.disease Hsp70 Rats medicine.anatomical_structure Phlorhizin chemistry Adipose Tissue Animals Newborn embryonic structures cardiovascular system |
| Zdroj: | The Journal of endocrinology. 190(2) |
| ISSN: | 0022-0795 |
| Popis: | The 70 kDa heat shock protein family plays important cardiac protective roles against myocardial injuries. Reduced myocardial protection is a common feature of diabetic myocardium. This study was carried out to define the changes in the 70 kDa heat shock protein family in the myocardium in the of streptozotocin-diabetes rats, and to explore the mechanisms through which diabetes alters the abundance of Hsp70/Hsc70 in cardiac muscle. In the diabetic myocardium, the abundance of Hsc70 was significantly reduced. The abundance of Hsp70 was low in cardiac muscle and was not induced in the diabetic myocardium. Unlike Hsp60, Hsp70 and Hsc70 did not augment insulin-like growth factor-I receptor signaling in cardiac muscle cells. In cultured cardiomyocytes, insulin directly increased the abundance of Hsc70, whereas insulin could not modulate Hsp70. Treating diabetic rats with insulin restored myocardial Hsc70 level, but phlorizin treatment failed to restore myocardial Hsc70. These in vivo and in vitro studies showed that downregulation of Hsc70 in diabetic myocardium was secondary to insulin deficiency. Thus, insulin played a major role in maintaining adequate expression of Hsc70 in cardiac muscle. |
| Databáze: | OpenAIRE |
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