Molecular Cloning and Expression of the Type 1 and Type 2 Murine Receptors for Tumor Necrosis Factor
| Autor: | Craig A. Smith, Raymond G. Goodwin, Nancy A. Jenkins, Neal G. Copeland, Dirk K. Anderson, Terri Davis, Camilynn I. Brannan, Rita Jerzy |
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| Rok vydání: | 1991 |
| Předmět: |
T-Lymphocytes
Molecular Sequence Data Gene Expression Receptors Cell Surface Saccharomyces cerevisiae Molecular cloning Biology Transfection Receptors Tumor Necrosis Factor Homology (biology) Cell Line Mice Cell surface receptor Sequence Homology Nucleic Acid Animals Humans Genomic library Amino Acid Sequence Cloning Molecular Receptor Gene Peptide sequence Molecular Biology Gene Library Tumor Necrosis Factor-alpha Chromosome Mapping Cell Biology Molecular biology Recombinant Proteins Kinetics Tumor necrosis factor alpha Research Article |
| Zdroj: | Molecular and Cellular Biology. 11:3020-3026 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.11.6.3020-3026.1991 |
| Popis: | Clones encoding the type 1 (p80) and type 2 (p60) forms of the murine receptors for tumor necrosis factor (TNF) were isolated by cross-hybridization using probes derived from the cloned human TNF receptors. Each of the murine receptors shows strong sequence homology to the corresponding human receptor (approximately 65% amino acid identity) throughout the molecule but only modest homology, limited to ligand-binding domains, between themselves. The ligand-binding characteristics of the recombinant murine receptors mirror those of the human homologs: both receptor types bind TNF-alpha and -beta with multiple affinity classes, and the ligands cross-compete. Analysis of the murine transcripts encoding these receptors revealed the presence of RNAs for one or both forms of the receptors in all cells examined. It was also demonstrated that for both types of human TNF receptor, variably sized transcripts are observed in different cells. The murine cDNAs were further used to determine the chromosomal locations of the TNF receptor genes. They are not linked, in contrast to the ligands, and map to chromosomes 4 (type 1) and 6 (type 2). |
| Databáze: | OpenAIRE |
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