Hyaluronidase decreases neutrophils infiltration to the inflammatory site
| Autor: | Francisco Wanderley Garcia Paula-Silva, Cornélia Muhr, Stephen F. Rodrigues, Irmgard Merfort, Lúcia Helena Faccioli, Marcio Fronza, Denise Sayuri Calheiros da Silveira, Sandra Helena Poliselli Farsky, Carlos Arterio Sorgi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Leukotriene B4 Immunology Anti-Inflammatory Agents Hyaluronoglucosaminidase Inflammation Carrageenan CITOCINAS Andrology Leukocyte Count 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Hyaluronidase Cell Adhesion Leukocytes medicine Animals Leukocyte Rolling Cell adhesion Pharmacology medicine.disease Mice Inbred C57BL 030104 developmental biology chemistry 030220 oncology & carcinogenesis Blood Vessels Cytokines Tumor necrosis factor alpha medicine.symptom Infiltration (medical) Intravital microscopy medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | To evaluate the in vivo anti-inflammatory potential of bovine hyaluronidase (HYAL) using two different models of acute inflammation. Air pouches were produced in the dorsal subcutaneous of mice and injected with phosphate saline solution or HYAL. The antiinflammatory action of HYAL was evaluated in carrageenan (Cg)-inflamed air pouches. After 4 and 24 h the cellular influx, protein exudation, cytokines and lipid mediators were evaluated. The action of HYAL on the rolling and adhesion of leukocytes was investigated in the LPS-stimulated mesenteric microcirculation by intravital microscopic. Treatment with HYAL reduced the cellular influx and protein exudation in non-inflamed and inflamed air pouches. HYAL treatment of Cg-inflamed air pouch reduced the production of tumor necrosis factor-alpha (TNF-α), interleukin-8 (IL-8), leukotriene B4 (LTB4) and LTC4, whereas prostaglandins E2 (PGE2) and D2 (PGD2) concentrations were unchanged. Histological analyses showed that HYAL administration diminished cell infiltration in the air-pouch lining. In LPS-stimulated mesenteric microcirculation, HYAL usage decreased rolling and adhesion of leukocytes, but did not affect the blood vessels diameters. The results demonstrate that HYAL inhibited cellular recruitment, edema formation and pro-inflammatory mediators production, resulting in decreased adherence of leukocytes to blood vessels and tissue infiltration. Our data suggest that HYAL may be considered an effective candidate to ameliorate acute inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink