T Cell Phenotype in Allergic Asthma and Atopic Dermatitis
| Autor: | Carsten B. Schmidt-Weber, Werner Kneist, Günther Menz, Steffen Kunzmann, Cezmi A. Akdis, Jan G. Wohlfahrt, Kurt Blaser |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Allergy T cell Immunology Biology Polymerase Chain Reaction Dermatitis Atopic Chemokine receptor Transforming Growth Factor beta Gene expression medicine Humans Immunology and Allergy Receptor Oligonucleotide Array Sequence Analysis Endothelin-3 Interleukin-13 Tissue Inhibitor of Metalloproteinases General Medicine Atopic dermatitis medicine.disease Phenotype Asthma Matrix Metalloproteinases medicine.anatomical_structure Gene Expression Regulation biology.protein Cytokines RNA Receptors Chemokine Interleukin-4 |
| Zdroj: | International Archives of Allergy and Immunology. 131:272-282 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000072139 |
| Popis: | Background: T cells are key regulators of immunologic disease parameters. However, their contribution to the process of tissue remodeling is ill defined. In the present study, we investigated gene expression of allergy-characteristic, IL-4-rich T cell cDNAs to monitor expression of genes that might participate in the pathogenesis of allergic diseases. Methods: cDNAs of freshly isolated and restimulated CD4+ T cells from patients with allergic asthma (AA) or atopic dermatitis (AD) and healthy subjects were analyzed on Nylon membrane-based DNA arrays. Three patients were selected for an allergy-characteristic T cell phenotype with high IL-4 expression (AA) or IL-13 expression (AD). Results: Several gene families such as the TGF-β family, chemokines and chemokine receptors were found to be upregulated. Matrix metalloproteinases and their inhibitors were also found to be expressed in an enhanced manner. Furthermore, factors regulating tissue turnover such as fibroblast growth factors and neurotrophic as well as vasoactive factors were found be expressed at a higher level in allergic patient compared to healthy donors. Conclusion: The present study reveals and confirms genes relevant for allergy and highlights an approach to applying a DNA array technique for diagnostic discrimination of allergic diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|