Ex vivo expansion of hematopoietic stem cells
| Autor: | Cheng Cheng Zhang, JingJing J. Xie |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cellular differentiation
Cell Culture Techniques Bone Marrow Cells Mice SCID Biology General Biochemistry Genetics and Molecular Biology Mice Environmental Science(all) Animals Humans Cell Lineage Clustered Regularly Interspaced Short Palindromic Repeats General Environmental Science Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Genetic Therapy Fetal Blood Hematopoietic Stem Cells Coculture Techniques Cell biology Perfusion Transplantation Haematopoiesis Cord blood Immunology SCID-Repopulating Cell Stromal Cells Stem cell General Agricultural and Biological Sciences Signal Transduction Homing (hematopoietic) |
| Zdroj: | Science China Life Sciences. 58(9):839-853 |
| ISSN: | 1674-7305 |
| DOI: | 10.1007/s11427-015-4895-3 |
| Popis: | Ex vivo expansion of hematopoietic stem cells (HSCs) would benefit clinical applications in several aspects, to improve patient survival, utilize cord blood stem cells for adult applications, and selectively propagate stem cell populations after genetic manipulation. In this review we summarize and discuss recent advances in the culture systems of mouse and human HSCs, which include stroma/HSC co-culture, continuous perfusion and fed-batch cultures, and those supplemented with extrinsic ligands, membrane transportable transcription factors, complement components, protein modification enzymes, metabolites, or small molecule chemicals. Some of the expansion systems have been tested in clinical trials. The optimal condition for ex vivo expansion of the primitive and functional human HSCs is still under development. An improved understanding of the mechanisms for HSC cell fate determination and the HSC culture characteristics will guide development of new strategies to overcome difficulties. In the future, development of a combination treatment regimen with agents that enhance self-renewal, block differentiation, and improve homing will be critical. Methods to enhance yields and lower cost during collection and processing should be employed. The employment of an efficient system for ex vivo expansion of HSCs will facilitate the further development of novel strategies for cell and gene therapies including genome editing. |
| Databáze: | OpenAIRE |
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