Determination of artemisinin and its analogs in Artemisia annua extracts by capillary electrophoresis - Mass spectrometry
| Autor: | Attila Gáspár, Cynthia Nagy, Gábor Vasas, Melinda Andrási, Anna Pesti |
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| Rok vydání: | 2021 |
| Předmět: |
Formic acid
Clinical Biochemistry Artemisia annua Pharmaceutical Science 01 natural sciences Capillary electrophoresis–mass spectrometry Micellar electrokinetic chromatography Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Drug Discovery medicine Artemisinin Spectroscopy Chromatography biology 010405 organic chemistry Plant Extracts 010401 analytical chemistry Electrophoresis Capillary biology.organism_classification Artemisinins 0104 chemical sciences chemistry Casticin Ascaridole medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 202 |
| ISSN: | 1873-264X |
| Popis: | The applicability of micellar electrokinetic capillary chromatography (MEKC) with mass spectrometric detection for the determination of artemisinin and its analogs (e.g. ascaridole, artemisia ketone, casticin, deoxyartemisinin, arteannuic acid, artemetin, dihydroartemisinic acid) was studied. 40 mM ammonium perfluorooctanoate (pH 9.5) with 2% isopropanol (IPA) was used as background electrolyte (BGE) and the sheath liquid was 50 % (v/v) IPA:water containing 0.1 % formic acid. Separation was performed in a bare fused silica capillary. Artemisinin was detected at 283.1545 m/z as [M+H]+ ion. For artemisinin the linear range was found to be 0.6 μg/mL - 60 μg/mL and the limit of detection was 0.18 μg/mL. The RSD% values were 2.6 % for migration times and 4.8 % for peak areas (N = 6). In the ethanolic extracts of Artemisia annua leaves, in addition to artemisinin, a large number of other organic components could be separated and determined. MEKC-MS revealed the existence of diastereomers of several compounds (artemisinin, deoxyartemisinin, dihydroartemisinic acid) in the plant extracts. |
| Databáze: | OpenAIRE |
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