The yeast 14-3-3 proteins BMH1 and BMH2 differentially regulate rapamycin-mediated transcription

Autor: Emily L. Humphrey-Dixon, Hunter L. Berrus, Jonathan R. Whicher, Michael A. Trembley
Rok vydání: 2013
Předmět:
Cell signaling
Saccharomyces cerevisiae Proteins
Transcription
Genetic
NCR
nitrogen catabolite repression
Biophysics
lcsh:Life
lcsh:QR1-502
Ribosome biogenesis
ribosome biogenesis
Saccharomyces cerevisiae
Biology
Biochemistry
S2
lcsh:Microbiology
Bmh2
03 medical and health sciences
0302 clinical medicine
Transcription (biology)
Molecular Biology
Gene
Psychological repression
14-3-3
030304 developmental biology
GO
gene ontology
Genetics
Sirolimus
target of rapamycin (TOR)
0303 health sciences
Original Paper
rapamycin
Promoter
TOR
target of rapamycin
Cell Biology
WT
wild-type
Yeast
3. Good health
Cell biology
ChIP
chromatin immunoprecipitation
lcsh:QH501-531
14-3-3 Proteins
nitrogen catabolite repression (NCR)
Nitrogen catabolite repression
qPCR
quantitative PCR
030217 neurology & neurosurgery
WCE
whole-cell extract
Zdroj: Bioscience Reports
Bioscience Reports, Vol 34, Iss 2, p e00099 (2014)
ISSN: 1573-4935
Popis: 14-3-3 proteins are highly conserved and have been found in all eukaryotic organisms investigated. They are involved in many varied cellular processes, and interact with hundreds of other proteins. Among many other roles in cells, yeast 14-3-3 proteins have been implicated in rapamycin-mediated cell signalling. We determined the transcription profiles of bmh1 and bmh2 yeast after treatment with rapamycin. We found that, under these conditions, BMH1 and BMH2 are required for rapamycin-induced regulation of distinct, but overlapping sets of genes. Both Bmh1 and Bmh2 associate with the promoters of at least some of these genes. BMH2, but not BMH1, attenuates the repression of genes involved in some functions required for ribosome biogenesis. BMH2 also attenuates the activation of genes sensitive to nitrogen catabolite repression.
Two yeast 14-3-3 proteins, were assumed to have redundant functions because they have nearly identical sequences. Here, we provide evidence that they differentially regulate the transcription of genes in response to inhibition of the TOR pathway by rapamycin treatment.
Databáze: OpenAIRE
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