Synthetic and immunological studies of N-acyl modified S-linked STn derivatives as anticancer vaccine candidates
| Autor: | Chang-Cheng Liu, Xiu-Jing Zheng, Xin-Shan Ye, An Xiao, Chang-Xin Huo, Shuang Sun, Zhuo Lv |
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| Rok vydání: | 2015 |
| Předmět: |
Male
chemistry.chemical_classification Mice Inbred BALB C Glycan biology Glycoconjugate Carbohydrate chemistry Immunogenicity Organic Chemistry Cancer Vaccines Biochemistry Antigen chemistry biology.protein Animals Antigens Tumor-Associated Carbohydrate Female Immunization Physical and Theoretical Chemistry Antibody Glycoconjugates Keyhole limpet hemocyanin Conjugate |
| Zdroj: | Organic & Biomolecular Chemistry. 13:3677-3690 |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/c4ob02424a |
| Popis: | It is well known that tumor cells express some aberrant glycans, termed tumor-associated carbohydrate antigens (TACAs). TACAs are good targets for the development of carbohydrate-based anticancer vaccines. However, one of the major problems is that carbohydrate antigens possess a weak immunogenicity. To tackle this problem, a number of unnatural N-modified S-linked STn analogues were designed and prepared. Reaction of the modified STn disaccharides with bifunctional adipic acid p-nitrophenyl diester provided the corresponding activated esters, which was followed by the conjugation with keyhole limpet hemocyanin (KLH), affording the corresponding protein conjugates. The immunological properties of these glycoconjugates were evaluated in a mouse model. The results showed that the modified glycoconjugates stimulated the production of IgG antibodies that are capable of recognizing the naturally occurring STn antigen, helping the discovery of carbohydrate-based anticancer vaccine candidates. |
| Databáze: | OpenAIRE |
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