False-positive SARS-CoV-2 serology in 3 children with Kawasaki disease

Autor: Ka Li Kwok, Wilfred Hs Wong, Kwok-Hung Chan, Keith Ts Tung, Joshua Sc Wong, Yuen Yu Lam, Ian C. K. Wong, Godfrey Cf Chan, Xue Li, Celine Sl Chui, Christina Sm Wong, Dennis Chi Yu Au, Kelvin K. W. To, Marco Hk Ho, Kwok-Yung Yuen, Winnie Wy Tso, Gilbert T. Chua, Carol Hy Fong, Patrick Ip, Mike Yw Kwan, Rosa S. Wong
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Antibodies
Viral
Polymerase Chain Reaction
Serology
0302 clinical medicine
030212 general & internal medicine
False positive
Child
Immunoassay
Aspirin
medicine.diagnostic_test
biology
General Medicine
Nucleocapsid Proteins
Infectious Diseases
Molecular Diagnostic Techniques
Spike Glycoprotein
Coronavirus
Hong Kong
Antibody
Coronavirus Infections
Systemic vasculitis
medicine.drug
Microbiology (medical)
Pneumonia
Viral
030106 microbiology
Mucocutaneous Lymph Node Syndrome
Article
Betacoronavirus
03 medical and health sciences
medicine
Coronavirus Nucleocapsid Proteins
Humans
Microneutralization Assay
False Positive Reactions
Serologic Tests
Pandemics
Retrospective Studies
Chinese
Kawasaki disease
SARS-CoV-2
business.industry
COVID-19
Outbreak
Phosphoproteins
medicine.disease
Antibodies
Neutralizing
Immunology
biology.protein
business
Zdroj: Diagnostic Microbiology and Infectious Disease
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2020.115141
Popis: Background Kawasaki disease (KD) is an acute febrile and eruptive disease with systemic vasculitis predominantly affecting young East Asian children. Recent reports showed that children with KD-like disease from KD low prevalence regions had positive SARS-CoV-2 serology despite a negative SARS-CoV-2 polymerase chain reaction (PCR) in respiratory samples. Objectives To describe three pediatric Kawasaki Disease patients with false positive SARS-CoV-2 serology. Study design We retrospectively recruited children with KD diagnosed during the COVID-19 outbreak in Hong Kong. Clinical characteristics and laboratory test results including SARS-CoV-2 PCR results were retrieved. We performed a microparticle-based immunoassay for the detection of IgG against nucleoprotein (NP) and spike protein receptor binding domain (RBD), and a microneutralization assay for the detection of neutralizing antibodies. Results Three Chinese children with typical KD were identified. They had no epidemiological links with COVID-19 patients and tested negative for SARS-CoV-2 NPA PCR. They were treated with IVIG and aspirin, and were discharged without complications. Subsequently two of them were tested positive against anti-RBD and anti-NP antibodies and one was tested positive against anti- RBD antibodies. However, microneutralization assay showed that neutralizing antibodies were absent, suggesting a false-positive IgG result. Conclusion Detection of neutralizing antibodies is recommended to confirm previous SARS-CoV-2 infection in IgG-positive but PCR-negative patients.
Databáze: OpenAIRE
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