Reparative effects of neural stem cells in neonatal rats with hypoxic–ischemic injury are not influenced by host sex
Autor: | Richard E. Hartman, Evan Y. Snyder, Christopher M. Denham, Melissa S. Dulcich, Christine I. Turenius, Andre Obenaus, Stephen Ashwal, Beatriz Tone, Nirmalya Ghosh |
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Rok vydání: | 2014 |
Předmět: |
Male
medicine.medical_specialty Pathology Brain development Biology Ferric Compounds Pediatrics Regenerative medicine Paediatrics and Reproductive Medicine 03 medical and health sciences Sex Factors 0302 clinical medicine Neural Stem Cells Hypoxia-Ischemia medicine Animals 030304 developmental biology Hypoxic ischemic Behavior 0303 health sciences medicine.diagnostic_test Animal Brain Magnetic resonance imaging Magnetic Resonance Imaging Neural stem cell Rats 3. Good health Pediatrics Perinatology and Child Health Cerebral ventricle Public Health and Health Services Female Histopathology Stem cell 030217 neurology & neurosurgery Stem Cell Transplantation |
Zdroj: | Pediatric research, vol 75, iss 5 |
ISSN: | 1530-0447 0031-3998 |
Popis: | BackgroundGender is increasingly recognized as an important influence on brain development, disease susceptibility, and response to pharmacologic/rehabilitative treatments. In regenerative medicine, it remains entirely unknown whether there is an interaction between transplanted stem cells and host gender that might bias efficacy and safety in some patients but not others.MethodsWe examined the role of recipient gender in a neonatal rat hypoxic-ischemic injury (HII) model, treated with female human neuronal stem cells (hNSCs), labeled with superparamagnetic iron oxide particles implanted into the contralateral cerebral ventricle. We monitored HII evolution (by magnetic resonance imaging, histopathology, behavioral testing) and hNSC fate (migration, replication, viability).ResultsRecipient gender after implantation did not influence the volume or location of ischemic injury (1, 30, or 90 d) or behavior (90 d). Superparamagnetic iron oxide labeling did not influence HII evolution. Implantation had its greatest benefit on mild/moderate injuries, which remained stable rather than increasing as in severe HII as is the natural history for such lesions.ConclusionOur results suggest that hNSC treatment (including using hNSCs that are prelabeled with iron to allow tracking in real time by magnetic resonance imaging) would be equally safe and effective for male and female human newborns with mild-to-moderate HII. |
Databáze: | OpenAIRE |
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