The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis

Autor: Andrés E. Carrasco, Ana M. Adamo, Martin M. Riccomagno, Eduardo M. Castaño, Mikio Shoji, Oscar H. Ocaña, Laura Morelli, Alejandra R. Paganelli, Paula G. Franco, Silvia L. López, José L. Affranchino, Etsuro Matsubara, María I. Prat
Rok vydání: 2001
Předmět:
Central Nervous System
Embryology
Embryo
Nonmammalian
Xenopus
Apoptosis
Xenopus Proteins
Xenopus laevis
Tubulin
Aspartic Acid Endopeptidases
Sonic hedgehog
In Situ Hybridization
Neurons
Reverse Transcriptase Polymerase Chain Reaction
Neurogenesis
Gene Expression Regulation
Developmental
Cell Differentiation
DNA-Binding Proteins
embryonic structures
Neural plate
Cell Division
Signal Transduction
animal structures
Kruppel-Like Transcription Factors
Down-Regulation
Nerve Tissue Proteins
Tretinoin
Biology
ZIC2
Presenilin
Zinc Finger Protein Gli3
Endopeptidases
mental disorders
GLI3
Presenilin-1
Animals
Hedgehog Proteins
RNA
Messenger
Floor plate
Membrane Proteins
Oligonucleotides
Antisense
biology.organism_classification
nervous system diseases
Repressor Proteins
nervous system
Mutagenesis
Site-Directed
Trans-Activators
Cancer research
biology.protein
Amyloid Precursor Protein Secretases
Transcription Factors
Developmental Biology
Zdroj: Mechanisms of Development. 107:119-131
ISSN: 0925-4773
DOI: 10.1016/s0925-4773(01)00458-0
Popis: We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin 1 primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-a morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. q 2001 Elsevier Science Ireland Ltd. All rights reserved.
Databáze: OpenAIRE
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