Production and purification of large amounts of Rous sarcoma virus
| Autor: | Ralph E. Smith, Eugene H. Bernstein |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Sucrose
animal structures Time Factors Virus Cultivation viruses Optical density measurement Chick Embryo Tritium Virus Replication General Biochemistry Genetics and Molecular Biology Virus Equilibrium density chemistry.chemical_compound Glycols Culture Techniques Centrifugation Density Gradient Virology and Viral Immunology Animals Chemical Precipitation Centrifugation General Pharmacology Toxicology and Pharmaceutics Infectious virus Fucose Glycoproteins Rous sarcoma virus Chromatography General Immunology and Microbiology biology Proteins General Medicine Fibroblasts biology.organism_classification Molecular biology Cell Transformation Neoplastic chemistry Avian Sarcoma Viruses Ammonium Sulfate Viruses Electrophoresis Polyacrylamide Gel Peptides |
| Zdroj: | Applied microbiology. 25(3) |
| ISSN: | 0003-6919 |
| Popis: | Procedures are described for production and purification of large amounts of Rous sarcoma virus. The virus was produced by Rous sarcoma virus-transformed chicken embryo fibroblasts in roller culture which produced up to 6 mg of virus per day per liter of supernatant fluid. Various methods of concentrating virus were evaluated; pelleting yielded the best results in terms of recovery of infectious virus. Purification was achieved by means of successive velocity and equilibrium density centrifugation by using sucrose solutions made in low-salt buffer. A rapid method for the optical density measurement of virus concentration was also developed. |
| Databáze: | OpenAIRE |
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