Effects of Dietary Oat Beta-Glucans on Colon Apoptosis and Autophagy through TLRs and Dectin-1 Signaling Pathways—Crohn’s Disease Model Study
| Autor: | Małgorzata Gajewska, Katarzyna Dziendzikowska, Łukasz Kopiasz, Michał Oczkowski, Joanna Gromadzka-Ostrowska, Kinga Majchrzak-Kuligowska, Tomasz Królikowski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Crohn’s disease beta-Glucans colitis Rats Sprague-Dawley 0302 clinical medicine Crohn Disease oat beta-glucan Intestinal Mucosa Receptor Gastrointestinal tract Nutrition and Dietetics medicine.diagnostic_test Caspase 3 Toll-Like Receptors apoptosis 030220 oncology & carcinogenesis Cytokines medicine.symptom Signal transduction Microtubule-Associated Proteins lcsh:Nutrition. Foods and food supply Dectin-1 Signal Transduction medicine.medical_specialty autophagy Colon Inflammation lcsh:TX341-641 Biology Article 03 medical and health sciences Western blot Internal medicine medicine Animals Lectins C-Type Colitis TLRs Autophagy medicine.disease Rats Disease Models Animal 030104 developmental biology Endocrinology Apoptosis Dietary Supplements Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 321, p 321 (2021) Nutrients Volume 13 Issue 2 |
| ISSN: | 2072-6643 |
| Popis: | Background: Crohn’s disease (CD) is characterized by chronic inflammation of the gastrointestinal tract with alternating periods of exacerbation and remission. The aim of this study was to determine the time-dependent effects of dietary oat beta-glucans on colon apoptosis and autophagy in the CD rat model. Methods: A total of 150 Sprague–Dawley rats were divided into two main groups: healthy control (H) and a TNBS (2,4,6-trinitrobenzosulfonic acid)-induced colitis (C) group, both including subgroups fed with feed without beta-glucans (βG−) or feed supplemented with low- (βGl) or high-molar-mass oat beta-glucans (βGh) for 3, 7, or 21 days. The expression of autophagy (LC3B) and apoptosis (Caspase-3) markers, as well as Toll-like (TLRs) and Dectin-1 receptors, in the colon epithelial cells, was determined using immunohistochemistry and Western blot. Results: The results showed that in rats with colitis, after 3 days of induction of inflammation, the expression of Caspase-3 and LC3B in intestinal epithelial cells did not change, while that of TLR 4 and Dectin-1 decreased. Beta-glucan supplementation caused an increase in the expression of TLR 5 and Dectin-1 with no changes in the expression of Caspase-3 and LC3B. After 7 days, a high expression of Caspase-3 was observed in the colitis-induced animals without any changes in the expression of LC3B and TLRs, and simultaneously, a decrease in Dectin-1 expression was observed. The consumption of feed with βGl or βGh resulted in a decrease in Caspase-3 expression and an increase in TLR 5 expression in the CβGl group, with no change in the expression of LC3B and TLR 4. After 21 days, the expression of Caspase-3 and TLRs was not changed by colitis, while that of LC3B and Dectin-1 was decreased. Feed supplementation with βGh resulted in an increase in the expression of both Caspase-3 and LC3B, while the consumption of feed with βGh and βGl increased Dectin-1 expression. However, regardless of the type of nutritional intervention, the expression of TLRs did not change after 21 days. Conclusions: Dietary intake of βGl and βGh significantly reduced colitis by time-dependent modification of autophagy and apoptosis, with βGI exhibiting a stronger effect on apoptosis and βGh on autophagy. The mechanism of this action may be based on the activation of TLRs and Dectin-1 receptor and depends on the period of exacerbation or remission of CD. |
| Databáze: | OpenAIRE |
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