Heterologous expression, purification, and phylogenetic analysis of oil-degrading biosurfactant biosynthesis genes from the marine sponge-associated Bacillus licheniformis NIOT-06
| Autor: | Lawrance Anburajan, Ramalingam Kirubagaran, P.S. Dheenan, Nambali Valsalan Vinithkumar, Divya Shridhar, Toms C. Joseph, Balakrishnan Meena, Rangamaran Vijaya Raghavan, Gopal Dharani |
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| Rok vydání: | 2014 |
| Předmět: |
Bioengineering
Bacillus Marine Biology Microbiology chemistry.chemical_compound Surface-Active Agents Biosynthesis Spectroscopy Fourier Transform Infrared Transferase Animals Bacillus licheniformis Chromatography High Pressure Liquid Phylogeny chemistry.chemical_classification Chromatography Reverse-Phase biology Lipopeptide Fatty acid General Medicine biology.organism_classification Amino acid Porifera Biodegradation Environmental chemistry Biochemistry Genes Bacterial Heterologous expression Surfactin Oils Biotechnology |
| Zdroj: | Bioprocess and biosystems engineering. 38(6) |
| ISSN: | 1615-7605 |
| Popis: | Surfactin is a lipopeptide, composed of one β-hydroxy fatty acid, a long fatty acid moiety, and seven amino acids. In this study, the biosurfactant biosynthesis genes; 4′-pantetheinyl transferase (sfp), phosphopantetheinyl transferase (sfpO), and surfactin synthetase (srfA) have been characterized from the marine sponge-associated Bacillus licheniformis NIOT-06 from the Andaman and Nicobar Islands. The purified recombinant biosurfactant revealed excellent emulsification activity with crude oil and kerosene. Reverse-phase high-performance liquid chromatography resolved the purified recombinant biosurfactant into several fractions and one of which had significant surface tension reducing property. Fourier transform infrared spectroscopy spectrum also revealed the presence of C–N–N, alkenes, and N–H as the functional groups, and a similar overlapping pattern was observed with that of standard lipopeptide surfactin. The diversity and phylogeny of sfp, sfpO, and srfA gene sequences were compared with other eubacteria. The sfp, sfpO, and srfA gene sequences obtained from Bacillus licheniformis NIOT-06 were diverse and appeared to be partially conserved when compared with the GenBank reported sequences of several eubacteria. |
| Databáze: | OpenAIRE |
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