Exploring SARS-COV-2 structural proteins to design a multi-epitope vaccine using immunoinformatics approach: An in silico study
| Autor: | Ali Rafiei, Maryam Ghasemi-Dehnoo, Morteza Alizadeh, Shahram Tahmasebian, Fatemeh Azadegan-Dehkordi, Korosh Ashrafi Dehkordi, Nader Bagheri, Mohammad-Hassan Arjmand, Samira Sanami, Masoud Nosrati, Hamed Nosrati |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
China Antigenicity COVID-19 Vaccines Helper T lymphocyte medicine.medical_treatment In silico Epitopes T-Lymphocyte Health Informatics Computational biology Biology Article Epitope 03 medical and health sciences 0302 clinical medicine Antigen medicine Humans SARS-CoV-2 Reverse vaccinology COVID-19 Computer Science Applications Molecular Docking Simulation CTL 030104 developmental biology CTxB Vaccines Subunit Epitopes B-Lymphocyte Vaccine Adjuvant 030217 neurology & neurosurgery |
| Zdroj: | Computers in Biology and Medicine |
| ISSN: | 0010-4825 |
| DOI: | 10.1016/j.compbiomed.2021.104390 |
| Popis: | In December 2019, a new virus called SARS-CoV-2 was reported in China and quickly spread to other parts of the world. The development of SARS-COV-2 vaccines has recently received much attention from numerous researchers. The present study aims to design an effective multi-epitope vaccine against SARS-COV-2 using the reverse vaccinology method. In this regard, structural proteins from SARS-COV-2, including the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins, were selected as target antigens for epitope prediction. A total of five helper T lymphocytes (HTL) and five cytotoxic T lymphocytes (CTL) epitopes were selected after screening the predicted epitopes for antigenicity, allergenicity, and toxicity. Subsequently, the selected HTL and CTL epitopes were fused via flexible linkers. Next, the cholera toxin B-subunit (CTxB) as an adjuvant was linked to the N-terminal of the chimeric structure. The proposed vaccine was analyzed for the properties of physicochemical, antigenicity, and allergenicity. The 3D model of the vaccine construct was predicted and docked with the Toll-like receptor 4 (TLR4). The molecular dynamics (MD) simulation was performed to evaluate the stable interactions between the vaccine construct and TLR4. The immune simulation was also conducted to explore the immune responses induced by the vaccine. Finally, in silico cloning of the vaccine construct into the pET-28 (+) vector was conducted. The results obtained from all bioinformatics analysis stages were satisfactory; however, in vitro and in vivo tests are essential to validate these results. |
| Databáze: | OpenAIRE |
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