Separation of biotin and its analogs by high-performance liquid chromatography: convenient labeling for ultraviolet or fluorimetric detection
| Autor: | Suzy Coustal, François Frappier, P.L. Desbene |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Chromatography
Biophysics Acetophenones Biotin Cell Biology medicine.disease_cause Coumarin Biochemistry Fluorescence High-performance liquid chromatography Sulfone Chromatographic separation chemistry.chemical_compound chemistry Coumarins Phase (matter) medicine Fluorometry Spectrophotometry Ultraviolet Molecular Biology Ultraviolet Chromatography High Pressure Liquid |
| Zdroj: | Analytical biochemistry. 128(2) |
| ISSN: | 0003-2697 |
| Popis: | d-Biotin, dethiobiotin, d-biotin sulfoxides, and d-biotin sulfone are converted to uv-absorbing or fluorescent derivatives by reaction with ω-4-dibromoacetophenone or 4-bromomethyl methoxy coumarin. High-performance liquid chromatographic separation conditions of these esters are described using C18 bonded phase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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