Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GST pi) and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas
| Autor: | Zhou Dc, Jean-Pierre Marie, Mariagrazia Michieli, Angela Michelutti, Michele Baccarani, R Fanin, Faussat Am, Daniela Damiani, R. Zittoun, Domenico Russo, C. Melli |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Cancer Research Lymphoma medicine.medical_treatment Gene Expression Drug resistance hemic and lymphatic diseases Gene expression medicine Tumor Cells Cultured Humans Gene Aged Glutathione Transferase Chemotherapy Leukemia biology Hematology Middle Aged medicine.disease Prognosis Drug Resistance Multiple Multiple drug resistance Glutathione S-transferase Oncology Immunology Cancer research biology.protein Female |
| Zdroj: | Leukemialymphoma. 15(5-6) |
| ISSN: | 1042-8194 |
| Popis: | By using RNA slot-blot technique, the frequency and the degree of GST pi and mdr-1 gene coexpression were investigated in 23 AML patients, 9 ALL, 9 CLL and 11 cases of NHL in an attempt to study their clinical and prognostic relevance. GST pi and mdr-1 levels were expressed as arbitrary units (U) with respect to the negative controls (U = 0), MCF7 and HL60 sensitive cell lines, and the positive controls (U = 10), MCF7/DOXO and HL60/DNR resistant cell lines. The concomitant GST pi/mdr-1 gene overexpression showed a negative prognostic value in the set of newly diagnosed AML pts (10 cases), furthermore higher GST pi and mdr-1 mRNA levels were averagely detected in the relapsed/resistant ALL pts (4 cases), and in CLL (7 cases) and NHL (8 cases) heavily pretreated patients who were unresponsive to chemotherapy and with a disease progression. These preliminary data show that two different mechanisms of drug resistance can be coexpressed at the same time in those leukemias and lymphomas with a clinically unfavourable course. |
| Databáze: | OpenAIRE |
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